冠状病毒Coronavirusl论文-2004 Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious periton.pdf

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1、VeterinaryMicrobiology99(2004)3142PrevalenceoffelinecoronavirustypesIandIIincatswithhistopathologicallyverifiedfelineinfectiousperitonitisVivianeBenetkaa,AnnaKübber-Heissb,JolantaKolodziejeka,NorbertNowotnya,d,MargareteHofmann-Parisotc,KarinMöstla,∗aClinicalVirologyGr

2、oup,InstituteofVirology,UniversityofVeterinaryMedicineVienna,Veterinärplatz1,A-1210Vienna,AustriabInstituteofPathologyandForensicVeterinaryMedicine,UniversityofVeterinaryMedicineVienna,Veterinärplatz1,A-1210Vienna,AustriacInstituteofMedicalPhysicsandBiostatistics,Uni

3、versityofVeterinaryMedicineVienna,Veterinärplatz1,A-1210Vienna,AustriadDepartmentofMedicalMicrobiology,FacultyofMedicineandHealthSciences,UnitedArabEmiratesUniversity,P.O.Box17666,AlAin,UnitedArabEmiratesReceived3December2002;receivedinrevisedform1July2003;accepted21

4、July2003AbstractFelinecoronaviruses(FCoV)varywidelyinvirulencecausingaspectrumofclinicalmanifestationsreachingfromsubclinicalcoursetofatalfelineinfectiousperitonitis(FIP).Independentofvirulencevariationstheyareseparatedintotwodifferenttypes,typeI,theoriginalFCoV,andt

5、ypeII,whichiscloselyrelatedtocaninecoronavirus(CCV).TheprevalenceofFCoVtypesinAustriancatpopulationswithoutFIPhasbeensurveyedrecentlyindicatingthattypeIinfectionspredominate.ThedistributionofFCoVtypesincats,whichhadsuccumbedtoFIP,however,wasfairlyunknown.PCRassayshav

6、ebeendevelopedamplifyingpartsofthespikeproteingene.Type-specificprimerpairsweredesigned,generatingPCRproductsofdifferentsizes.Atotalof94organpoolsofcatswithhistopathologicallyverifiedFIPwastested.Acleardifferentiationwasachievedin74cats,86%ofthemweretypeIpositive,7%typ

7、eIIpositive,and7%werepositiveforbothtypes.ThesefindingsdemonstratethatinFIPcasesFCoVtypeIpredominates,too,nonetheless,in14%ofthecasesFCoVtypeIIwasdetected,suggestingitscausativeinvolvementincasesofFIP.©2003ElsevierB.V.Allrightsreserved.Keywords:Felinecoronavirus(FCoV)

8、;Felineinfectiousperitonitis(FIP);RT-PCR;Paraffinembeddedtissues(PET);TypesIandII1.Introductionlidae.Thecausativeagent,thefelinecoro