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1、ARTICLESChineseScienceBulletin2004Vol.49No.6585ü590thefamilycoronaviridaeintheneworderNidovirales,hasasingle-strandedpositive-senseRNAgenome,whichisGenomesequencingand27.6kbinlength[2].ThegenomeofIBVhasmorethan10openreadingframes,whichencodetwopolyproteins
2、andcharacterizationanalysisofthreemajorstructuralproteinsandothersmallproteins.aBeijingisolateofchickenThreemajorstructuralproteinsarethespikeglycoprotein(S),themembraneglycoprotein(M),andthephosphory-coronavirusinfectiouslatednucleocapsidprotein(N).TheSpr
3、oteiniscleavedtoreleaseS1andS2proteinsaftertranslation.TheS1proteinbronchitisvirus[3ü5]inducesprotectiveimmunity.Andthemostantigenicofthevirusneutralizationantibody-inducingepitopesisJINWeiwu,CHENChen,ZHANGYing,formedbyafewaminoacidsthatoccurinthefirstandZ
4、HAOYiqiang,FENGJidong,CHENFuyong,thirdquartersoftheS1subunit,especiallyinthehyper-[6,7]WUQingming,YANGHanchun,WANGMing,variableregions.YUJialin,LINing,GONGYuanshi,SUNQixinIBVisthelonevirusintheantigenicgroupċof[8]&CHENZhangliangcoronaviridae,however,moreth
5、an20serotypeswithinIBVhavebeenrecognizedworldwideonthebasisofvi-AnimalCoronavirusResearchGroup,ChinaAgriculturalUniversity,[9]Beijing100094,Chinarus-neutralizationtest.Therefore,itisverydifficulttoCorrespondenceshouldbeaddressedtoLiNing(e-mail:ninglbau@pre
6、ventfromtheinfectionsofvirus.Thewholegenomeofpublic3.bta.net.cn)orChenZhangliang(e-mail:zhangliangchen@IBVwasdeterminedin1987[2].Andthegenomese-pku.edu.cn)quencesofIBVareveryimportantandusefulfordiseaseAbstractAvianinfectiousbronchitisvirus(AIBV)isdiagnosi
7、sandvaccination.However,remarkablevariationclassifiedasamemberofthegenuscoronavirusinthefamilywasobservedinvariousIBVisolates:thegenomeofIBVcoronaviridae.Theenvelopedvirushasapositive-sense,sin-isolateinthisregionmaybeverydifferentwiththatinthegle-stranded
8、RNAgenomeofapproximately28kilo-bases,otherregions.IBVBeijingisolate,whichcausedgreatwhichhasa5Ącapstructureand3Ąpolyadenylationtract.economiclosses,wasobtainedfromachickenhouseinThecompletegenomesequenceofinf