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1、JVetDiagnInvest20:28–32(2008)RespiratorydiseaseassociatedwithbovinecoronavirusinfectionincattleherdsinSouthernItaly1NicolaDecaro,MarcoCampolo,CostantinaDesario,FrancescoCirone,MariaD’Abramo,EleonoraLorusso,GraziaGreco,VivianaMari,MariaLoredanaColaianni,GabriellaElia,VitoMartella,CanioBu
2、onavogliaAbstract.Fouroutbreaksofbovinerespiratorydisease(BRD)associatedwithbovinecoronavirus(BCoV)infectioninItaliancattleherdswerereported.In3outbreaks,BRDwasobservedonlyin2–3-month-oldfeedlotcalves,whereasintheremainingoutbreak,lactatingcows,heifers,andcalvesweresimultaneouslyaffecte
3、d.Byusingreversetranscriptionpolymerasechainreaction(RT-PCR),BCoVRNAwasdetectedinalloutbreakswithoutevidenceofconcurrentviralpathogens(i.e.,bovinerespiratorysyncytialvirus,bovineherpesvirustype1,bovineviraldiarrheavirus,bovineparainfluenzavirus).Commonbacteriaofcattlewererecoveredonlyfr
4、om2outbreaksofBRD:Staphylococcusspp.andProteusmirabilis(outbreak1)andMannheimiahaemolytica(outbreak4).Arecentlyestablishedreal-timeRT-PCRassayshowedthatviralRNA27loadsinnasalsecretionsrangedbetween3.10310and7.50310RNAcopies/mloftemplate.Bovinecoronaviruswasisolatedfromrespiratoryspecime
5、nsfromalloutbreaksexceptoutbreak1,inwhichreal-timeRT-PCRfoundverylowviraltitersinnasalswabs.Keywords:Bovinecoronavirus;calves;Italy;respiratorydisease.IntroductiontationbecausetheshippingofcattlerepresentsastressingfactorthatmayfacilitatetheonsetofCoronaviruses(CoVs)arelarge,enveloped,s
6、ingle-BCoV-inducedrespiratorydisease,mainlyinfeedlotstrandedRNAvirusesthatareresponsibleforenteric,calves.Thepresentstudyreportstheresultsofrespiratory,orneurologicalsignsinmammalsandmolecularinvestigationsonoutbreaksofrespiratorybirds.BelongingtothefamilyCoronaviridaewithindiseasedueto
7、BCoVinfectioninItaliancattleherds.theorderNidovirales,CoVsareclassifiedinto3groupsonthebasisofantigenicandgeneticMaterialsandmethodsproperties.6Bovinecoronavirus(BCoV)isincludedOutbreaksandsamplecollectioningroup2atogetherwithmousehepatitisvirus,Outbreak1.InJanuary2006,respirat
