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1、第37卷第3期植物保护学报Vo.l37No.32010年6月ACTAPHYTOPHYLACICASINICAJune2010草莓枯萎病菌对多菌灵的抗性及其抗性菌株生物学特性1,2111*11顾春波史晓斌姜莉莉王开运林才华段海明(1.山东农业大学植物保护学院,泰安271018;2.枣庄出入境检验检疫局,枣庄277100)摘要:为评价草莓枯萎病菌对多菌灵的抗性风险,从山东有代表性的草莓种植区,采集、分离了4个草莓枯萎病菌菌株,分别在含多菌灵的PDA平板培养基上进行继代培养,诱导获得抗性菌株。采用菌落生长速率法测定抗
2、性菌株ZYD的抗性稳定性及其对其它杀菌剂的交互抗性,比较了抗性菌株ZYD与敏感菌株ZY的生物学特性差异。结果显示:选育至45代,抗性菌株ZYD对多菌灵的抗性达53.91倍,抗性能够在无药条件下稳定遗传,4个抗性菌株与敏感菌株ZY具有同样的致病力;抗多菌灵菌株ZYD对甲基硫菌灵等7种药剂表现出了不同程度的交互抗性;抗性菌株ZYD与敏感菌株ZY的适宜生长温度均为25,适宜pH均为9;在不同的碳、氮源条件下,ZYD与ZY的菌落直径和产孢量存在差异。关键词:草莓枯萎病;多菌灵;抗性;生物学特性ResistanceofFusarium
3、oxysporumf.sp.fragariaetocarbendazimandthebiologicalcharactersofcarbendazimresistantstrain1,2111*11GuChunboShiXiaobinJiangLiliWangKaiyunLinCaihuaDuanHaiming(1.CollegeofPlantProtection,ShandongAgriculturalUniversity,Taian271018,ShandongProvince,China;2.ZaozhuangEntry
4、ExitInspectionandQuarantineBureau,Zaozhuang277100,ShandongProvince,China)Abstract:FourisolatesofFusariumoxysporum.fsp.fragariaewerecollectedandisolatedfromstrawberryfieldsinShandongProvince,andselectedonthemediacontainingcarbendazimrespectively.ResistantstrainsofFusariu
5、moxysporum.fsp.fragariaewereobtainedafter45generationscultivation.ThebiologicalcharactersoftheresistantstrainZYDandsensitivestrainZYwerecompared,andthecrossresistanceofZYDtofungicideswasstudied.Theresultsshowedthatafter45generationsselection,theresistanceratioofZY
6、Dtocarbendazimreached53.91timesofthatofthesensitivestrain.TheresistantlevelofZYDaftersuccessiveincubationsonthefungicidefreeofPDAwasstable.Therewerenodifferencesbetweenresistantandsensitivestrainsinpathogeneticability.Meanwhile,positivecrossresistancetothiophanatemethy
7、land6otherfungicideswasfoundinZYD.ForthegrowthofZYandZYD,theoptimaltemperaturewas25,andtheoptimumpHvaluewas9.TheeffectsofdifferentcarbonandnitrogensourcesongrowthandsporeproductionofZYandZYDweredifferen.tKeywords:Fusariumoxysporum.fsp.fragariae;carbendazim;resistance
8、;biologicalcharacters基金项目:国家!十一五∀科技支撑计划(2006BAD08A03)作者简介:顾春波,男,1979年生,博士研究生,研究方向为农药毒理与有害生物抗药性,e
