低频电刺激双侧丘脑底核对癫痫模型大鼠脑内谷氨酸表达及海马细胞单位放电的影响.pdf

《低频电刺激双侧丘脑底核对癫痫模型大鼠脑内谷氨酸表达及海马细胞单位放电的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、第39卷第2期贵阳医学院学报Vo1．39No．22014年4月JOURNAL0FGUIYANGMEDICALCOLLEGE2014．4低频电刺激双侧丘脑底核对癫痫模型大鼠脑内谷氨酸表达及海马细胞单位放电的影响：l：游明瑶，伍国锋，蒋乃昌，王旭东(1．贵阳医学院附院神经科，贵州贵阳550004；2．贵阳医学院生理学教研室，贵州贵阳550004)[摘要]目的：观察低频电刺激(LFS)双侧丘脑底核(STN)对杏仁核电点燃癫痫模型大鼠脑内谷氨酸(Glu)表达及海马细胞单位放电(UCD)的影响。方法：健康

2、雄性Wistar大鼠30只随机分为对照组、癫痫模型组和LFS组，各组大鼠杏仁核均埋置刺激电极，对照组不刺激杏仁核，癫痫模型组及LFS组均电刺激制作癫痫模型。LFS组在癫痫点燃模型成功后予LFS双侧STN，观察各组大鼠癫痫样行为学改变，并记录海马UCD及脑组织Glu阳性神经元的表达。结果：癫痫模型组海马UCD频率较对照组增多(P<0．05)，LFS组海马UCD频率比模型组减少(P<0．05)；癫痫模型组脑组织中Glu表达较对照组增高(P<0．05)，LFS组脑组织中Glu的表达较癫痫模型组降低(P

3、<0．05)。结论：LFS双侧STN可抑制杏仁核电点燃癫痫模型大鼠脑内海马UCD频率及Glu阳性神经元的表达。[关键词]癫痫；大鼠，Wistar；电刺激疗法；丘脑底核；谷氨酸；低频；单位放电[中图分类号]R742．11；R454．1[文献标识码]A[文章编号]1000．2707(2014)02-0198-04InfluenceofLow-frequencyElectricalStimulationofBilateralSubthalamicNucleusontheGlutamateExpress

4、ioninBrainTissuesandUnitDischargesofHippocampalNeuronsofEpilepticModelRatsYOUMingyao，WUGuofeng，JIANGNaichang，WANGXudong(J．DepartmentofNeurology，theAffiliatedHospitalofGuiyangMedicalCollege，Guiyang550004，Gu&hou，China；2．Departmentofiology，GuiyangMedica

5、lCollege，Guiyang550004，Guizhou，China)[Abstract]0bjective：Toobservetheinfluenceoflow~equencyelectricalstimulation(LFS)ofbilat—eralsubthalamicnucleus(STN)onglutamate(Glu)expressioninbraintissuesandunitdischargesofhippocampalneurons(UCD)ofamygdaloidkind

6、lingepilepsyratmode1．Methods：ThirtyhealthymaleWistarratswererandomlydividedintocontrolgroup，epilepticmodelgroupandLFSgroup．Stimulatingelectrodeswereburiedinamygdaleofratsinthethreegroups，andamygdaleofratsinmodelgroupandLFSgroupwasstimulatedtoestablis

7、hepilepticmodel，thatofcontrolgroupwasnotstimulated．Aftermodeling，LFSgroupwasgivenLFStobilateralSTN．EpilepticsbehaviorsofratsineachgroupwereobservedandUCDwererecordedineachgroupandtheexpressionofGluwasdeter-mined．Results：UCD~equencyinepilepticmodelgro

8、upwasmorethanthatincontrolgroup，LFSgroupwaslessthanmodelgroup(P<0．05)．TheexpressionlevelofGluinepilepticmodelgroupwashigherthanthatincontrolgroup，LFSgroupwaslowerthanmodelgroup(P<0．05)．Conclu-sions：LFSmaysignificantlyinhibittheexpressionofglutamatean