跳跃性应力刺激对大鼠胫骨OPG、RANKL及骨代谢的影响.pdf

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1、542·中国运动医学杂志2014年6月第33卷第6期ChinJSpotsMed，Jun．2014，Vo1．33，No6·跳跃性应力刺激对大鼠胫骨OPG、RANKL及骨代谢的影响李盛村鲍捷王静王国祥苏州大学体育学院(江苏苏州215021)摘要目的：研究跳跃性应力刺激对大鼠骨组织的影响，探讨骨质破坏发生的分子生物学机制。方法：48只8周龄雄性SD大鼠，随机平均分为跳跃组和对照组，跳跃组按训练周数标记为J2、J4和J6组，相应对照组为C2、C4和C6组。跳跃组按计划进行跳跃运动训练，对照组安静饲养。每隔2周各组分别处死8只，检测血清碱性磷酸酶(ALP)和抗酒石酸酸性磷

2、酸(StrACP)，取一侧胫骨制作HE石蜡切片．另一侧胫骨应用RT—PCR法检测OPG、RANKLmRNA表达。结果：(1)与对照组相比，J6组骨胶原纤维和骨膜排列紊乱，骨皮质出现较大程度破坏，空腔增多。(2)3个跳跃组大鼠血清ALP和StrACP浓度均高于其对照组(P<0．05)。(3)J2组大鼠胫骨OPGmRNA表达量显著低于C2组(P<0．05)。而J6组OPGmRNA表达显著高于C6组(P<0．01)；J6组RANKLmRNA表达量显著高于C6组(P<0．01)。结论：过度跳跃性应力刺激后，大鼠胫骨OPG和RANKL表达增加，参与以骨转换率升高为特征的骨

3、质破坏过程。关键词跳跃应力：骨代谢；OPG／RANKLTheEfectofJumpingonTibialOPG，RANKLandBoneMetabolisminRatsLiShengcun，BaoJie，WangJing，WangGuoxiangSchooloficalEducation，SoochowUniversity，Jiangsu，China215021CorrespondingAuthor：WangGuoxiang，Email：kwang63@163．comAbstractobjectiveToinvestigateeffectofjumpingont

4、hebonetissueandexplorethemolecularmechanismofbonedestructioninrats．MethodsForty-eight8-weekoldSDratswereequallyrandomizedintojumpinggroupandcontrolgroup，and8ratsfromtwogroupswerekilledeverytwoweeksthereafter．Serumalkalinephosphatase(ALP)andanti-tartaricacidacidicphosphoricacid(StrACP

5、)weredetected．TibialmorphologywasobservedbyHEparaffinsection，andmRNAexpressionofosteoprotegerin(OPG)andreceptoractivatorofnuclearfactorkBligand(RANKL)byRT-PCR．Results(1)Disorderedperiostealcollagenfibers，damagedcorticalbone，andtheincreasedcavitywereseen6weeksafterjumpingascomparedwit

6、hcorrespondingcontrolgroup．(2)TheserumALPandStrACPlevelsfromthe2ndto6thweekafterjumpingweresignificantlyhigherthanthatinthecorespondingcontrolgroup(P<0．05)．(3)Theexpressionoftibia1OPGmRNA2weeksafterjumpingwaslowerthanthecorrespondingcontrolgroup(P

7、afterjumpingthanthatinthecorespondingcontrolgroup(P<0．O1)．ThemRNAexpressionofRANKLincreasedsignificantly6weeksafterjumpingascomparedwiththecorespondingcontrolgroup(P

8、dsjumpingstr