生物信息学题目.doc

《生物信息学题目.doc》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、第一次课1.PleasesearchonearticleviaPubMEDandgivetheabstract:LiuWY,etal.TheOsDHODH1geneisinvolvedinsaltanddroughttoleranceinrice.,JIntegrPlantBiol.2009Sep;51(9):825-33(OK)2.Pleasesearchthearticlesabouthumancancerresearchpublishedbefore2005.(OK)3.Pleasesearchallthearticlesrelatedtoriceyield,publishe

2、din“Nature”.(OK)4.Pleasesearchallliteraturespublishedfrom2000tonow,byauthorsfromNanjingAgriculturalUniversity.(OK)5.Pleasesearchallarticlesfromyourlaboratory,publishedin2010,andsavealltheabstractsinyourlocalcomputer.(OK)6.Pleasesearchliteraturesinvolvedinflowerdevelopment,writtenbyDr.Zhang,aft

3、er2002.(OK)第二次课1.SearchtheNRdatabasetochecktheZFP252paraloguesinriceandorthologuesinotherplantspeciesprotein中ZFP252，直接runblast2.SearchfordbESTdatabasetofindESTsofZFP252,thencheckthesourcesofZFP252expression.(OK)3.Searchforricezincfingerproteinsrelatedarticle(s)inPubMEDdatabase.（OK）1.Searchforr

4、icegeneZFP252inGenBank,givethegenelengthandnumberofaminoacids;CheckwhethersomeZFP252articleswerepublished.在基因库中搜寻ZFP252，基因长度和氨基酸数目，检测文章是否发表Nucleotide中ZFP2522.SearchUniGenedatabaseforricegeneZFP252andseekforthetissuesinwhichZFP252istranscribed.UNIGENE3.SeetheGEOprofileofZFP252andcheckwhatfactor

5、sregulateZFP252expression.U…GEO4.TrytodownloadthedataforsaltresponsivesmallRNAanalysisincottonandopenitinExcelprogram.GEODATESETSCotton[organism]ANDMiRNAANDsalt第三次课1.FindZFP252proteinsequencefromrice;从水稻中找到ZFP252蛋白序列2.RandomlypickfourZFP252-likeproteinsequencesfromvariousplantspecies;从各种植物物种中随

6、机选四种与ZFP252相似序列….RUNBLAST3.Multiplesequencealignmentforabove4-5sequences.CopythealignmentintoMs-WordorPPT.以上45序列序列多样性组合多序列比对1.FindriceZFP252sequenceandusethesequencetofindatleastthreedifferentZFP252-likeproteinsequencesindifferentplantspecies.ProteinZFP2522FindwhetherriceZFP182sequenceiscomple

7、te?FindatleastthreeESTsmatchingZFP182andshowtheESTsources.HowmanyZFP182-likemembersinricegenome?BLASTX找序列是否完整，找出至少三个EST相称的ZFP182，EST来源，在水稻基因组中找出与ZFP252相似成员NeclotideZFP182复制其DNA序列，找blast粘贴，选择EST数据再blast3Igotanewsequencefromriceasfollows: