行业标准：DB61∕T 413-2007 饮料中D一苹果酸的测定-酶法.pdf

《行业标准：DB61∕T 413-2007 饮料中D一苹果酸的测定-酶法.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、DB611if~1*DB61/T413-2007tI2007-09-03~~2007-09-03~~DB611T413-2007*tiFftJ:f:ll¥J~t~A~~*,H1~HjL*~ft~J:f:l~A~~~~M®mA~~.~~~.m*acQ*~ft~~~N:J:f:l~A~~~~~®mA~~.~~~Q*~ft~~A:~*~'~~*'*WW'~~~QI*tjf,ftJktt~~;fpQIIIiIII!i~iIj!I,~•J!l;i~IIDB61fT413-2007tftIl1;:$:tift/fUJI!

2、7l'if¢f~WlulE11A*4*D-:3jZ*mUfJ~iitl¥r1J~o*fiftttEmmTiX*4*D-1f:*~~itW!IJlEo2tJUiz31iJImjc14Lr:yux1I=*i¥J~~jffiJi*ti~ttEi¥JiJ!)..trn'fiJj~J~;:$:t;j;ttEi¥J,}~oJ'LM:r:EBjtJji¥JiJ!J+1~ft~:,;ltPiQfr§J'Ji:tHi1.a.(~~~~~i¥J~~)~.rr~~~mmT*~ttE,~W,.a.M*~ttE~~~~i¥J*~~~M

3、~"EJ1~ffli3:~X11:i¥J1iifA&;:$:ofLfik~ffBWHfJt}rm~fll:,;ltiid'#rA&;:$:JffJ::rFf;:$:flFttEoEN12138:;J.~*UJli~rtcpD-1j'i:~Il1tti¥Jl'if¢W!~1E.D-MDHD1j'Z*llil1i1i:lll?tI.~NAD:tj!JfiIi£~~~,*-A~1rllilNADHf3-':li!Iw:-~n~ut-'--':'i%tfllil~U~mIJ[~~lFJfl-f.fr.,if25·C

4、i¥JfijIt~14:ftJ=5}~1Ui¥J~m~it.$tfftPX;1~moli¥J~~0A11&7tf1tiEi:C~r9tR~*JJthepes4-f5:z..~IJJ&~z:.~jl'imlJI4JliHfD-1jL~~(D-~*~tik){tD1jL*~1i1i:~£~JW(D-MDH)Hi£i¥Jt~{5r.F,i!liJiNAD~f{:,7~~lP=mz..~tikolltmz..lliliiL0::liPEEffiJtfi¥J~{{tft?Hff.PX;jA]Iiil~1i1i:*11~

5、.:~ftfWt0JD-MDH,decarb)D-~*~1i1i:+NAD...p;jm1~~1i1i:+CO:c+NADH+H"IINADHi¥J,~,ffiD-1jL*~!Iii¥J,~,:!tit11:0INADHi¥J~id:rntE334,340!&365nm4}{!·r'i¥J1l'&7tJJ[{Ei:i¥J-'f:t>J~YJ3H~Lt5l"it1fIJfI5.1hepes5.2NADI5.3D-MDH!5.4NaClI!5.5KClIDB61!T413~20075.6Na2HP04.·2H2O

6、5.7lim'.5.8HCl5.9NaOH5.10KOH5.11:m:~*5.121Bj:ij"itffljij&litillj~6iii•6.1nRl#t(5mL,1mL50-200ILL10-50IlU6.2~t~.nn.::7:;~£1.0em6.3~91'~*:7:;Jlit,r:iJS£iJt*,tE340nmJIJ:AE6.4jJf:ll~~,11~1011m6.5J§iIL',ll6.6~IL'i~<~tf,~~jtILn;~*ft~G~;~~,J.&JIii~~7~jmIJ-WiJJ7.1

7、lit1f1J~ililj7.1.1hepes~;,*,j~1'£90mL:m:~*r:p:5t!tlIJ:JJO/.8gNaCL0.037gKCL0.0135gNazHPO,·2H20,0.1g1i1rfJ~,0.5ghepes,3'EJh~fft¥Jfi,J.j:jNaOH~~pH7.05,100mlo7.1.2NADI1-t:HiImLl1iJ~*mflllf.52.5rngf1)}&P:1JNADT1t:~,-15"C--20·C1:*tfo7.1.3D-MDHI1-t:Rl0.6mL.ffi:~

8、*?$M8UEr-Jf;f)}~P;k)D1ji:lti:l~Hl;:Jmtl.!W.I~1t~,-15'C--20·C1*#o7.1.4HCl:2mollL7.1.5NaOH:2mollL7.1.6KOH:2mollL7.2litt$ililji•7.2.11C~§t;~~~;tJ+jKOHllWl~25rnlm:HpHfll~7~8.:J.mm:l.J~iJj~.1im~.1lJ:H1.000L