耐碱转gsgsts基因苜蓿农艺性状调查

《耐碱转gsgsts基因苜蓿农艺性状调查》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、耐碱转GsGSTs基因苜蓿农艺性状调查　　摘要：为研究前期获得的耐碱转GsGSTs基因苜蓿与其对照在农艺性状上是否存在差异，进行了产量性状和形态性状的调查与统计分析。产量性状包括鲜草产量、干物质产量及茎分枝数；形态性状包括株高增长速度、节数及节间长度，同时进行了碱胁迫下表型分析。结果表明，转GsGSTs基因苜蓿植株在产量性状及形态性状方面与对照相比无显著差异。在碱胁迫下，非转基因苜蓿对照株系明显萎蔫、失绿，而转GsGSTs基因苜蓿株系均能保持较正常的生长状态。此结果说明外源基因GsGSTs在苜蓿中的表达没有改变其本身优良的农艺性状，同时其目

2、标性状（耐碱性）有所提高，符合转基因植物安全评价中“实质等同”原则。关键词：转基因苜蓿；GsGSTs基因；耐碱；农艺性状中图分类号：S541+.9文献标识码：A文章编号：0439-8114（2013）05-1105-04InvestigationandAnalysisontheAgronomicTraitsofAlkalineToleranceTransgenicAlfalfawithGsGSTsGeneWUJing，ZHUYan-ming，WANGZhen-yu，BAIXi，JIWei，CAIHua（PlantBio-Engineerin

3、gLaboratory，Collegeof3LifeSciences，NortheastAgriculturalUniversity，Harbin150030，China）Abstract：Inordertostudytheagronomicperformancedifferencesamongtransgenicalfalfalinesandthecontrol，theyieldtraits，morphologicaltraitsoftransgenicalfalfalineswereinvestigatedandanalyzed.The

4、yieldtraitsincludedthenumberofbranches，grassyieldanddryweight.Andthemorphologicaltraitsincludedthegrowthrateinspringandfall，plantheight，thenumberofnodesandthenodelength.AndthetransgenicalfalfalinesweretreatedwithNaHCO3.Theresultsshowedthattherewasnosignificantdifferencebet

5、weentransgenicalfalfalinesandnon-transgenicalfalfaplantsintheyieldtraitsandmorphologicaltraits.Anditwasfoundthattransgenicalfalfalinesgrewwellinthepresenceof100mmol/LNaHCO3，whilewild-typeplantsexpressedchlorosisandwiltingTheresultsindicatedthattheGsGSTsgenescouldenhancealk

6、alineresistanceanditsexpressioninalfalfahadnounfavorableeffectsinagronomictraits.Alltheresults3compliedwiththeprincipleof“substantialequivalence”oftransgenicplantsafetyevaluationKeywords：transgenicalfalfa；GsGSTsgene；tolerancetoalkaline-stress；agronomictraits3