最新外源雌激素处理对唐鱼卵黄蛋白原和雌激素受体基因表达...PPT课件.ppt

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1、外源雌激素处理对唐鱼卵黄蛋白原和雌激素受体基因表达...唐鱼(Tanichthysalbonubes)雄鱼雌鱼唐鱼唐鱼为鲤科(Cyprinidae)鱼丹亚科(Danioninae)唐鱼属(TanichthysLin,1932)仅分布于我国广州白云山一带山区小溪流中，濒危物种，属国家II级保护动物研究表明唐鱼对重金属Cu2+等敏感,是一种较理想的环境重金属污染指示生物目的意义克隆唐鱼VTG和雌激素受体基因分析E2对雄性唐鱼这些基因表达的影响方法基因克隆采用3’和5’RACE方法基因表达分析采用半定

2、量RT-PCR方法VTG:Nucleotideanddeducedaminoacidsequences1ACAAACCATCAGCAATGAGAGCTGTTGTGCTTGCCCCGACTGTAGCCCTTGTGGCGAGTC1MRAVVLAPTVALVASQ61AACAGATCAGCCTTGTTCCCGAGTTTGCCTCTGATAAGACCTATGTGTACAAGTATGAGG17QISLVPEFASDKTYVYKYEA121CTCTGCACTTGGGAGGACTTCCTCAAGAAGGTCT

3、GGCTAGAGTCGGAGCAAAAGTCAACG37LHLGGLPQEGLARVGAKVNG181GCAAGGTTTACATCCGTGCCGAGACAGAGAGCACCTTTCTGATGAAGTTCTCAGATGTTC57KVYIRAETESTFLMKFSDVQ241AACTCCATGAGTATACTGGCATTTGGCCCAGGGATCCTTTCGTTCCTGAAACTAAGCTCA77LHEYTGIWPRDPFVPETKLM301TGTCAACTATGACTGCTGAGCTGCAGATT

4、CCCATTAAGTTTGAGTATACCAATGGTGTGG97STMTAELQIPIKFEYTNGVV361TTGGAAAGATATTCGCCCCTGCAGGAGTCTCCCCTATTGTTCTGAACTTTTACAGAGGAA117GKIFAPAGVSPIVLNFYRGI421TCCTCAACATCTTTCAGCTTAACCTCAAGAAGACCCAGAACATCTATGAGCTGCAAGAGG137LNIFQLNLKKTQNIYELQEA481CTGGAGTTCAGGGAGTGTGCAG

5、GACCCACTATGTCATCAGTGAGGATTCGAAAGCCAACC157GVQGVCRTHYVISEDSKANH541ACATTACTGTCACCAAGTCTAAGGATCTGAGCCACTGCCAGGAGAGAATCACAAAAGATT177ITVTKSKDLSHCQERITKDF601TCGGCTTGGCATACACTGAGAAGTGTGACGAATGCACTGAGCGGGTAAAGAGTCTGATTG197GLAYTEKCDECTERVKSLIE661AAACCGCATCATAC

6、AACTACATCATGAAACCAACATCTGCCGGTGTATTGGTGTCTGAAG217TASYNYIMKPTSAGVLVSEA721CAACAGTTGAGGAAGTGTATCAGTTTTCACCATTCAATGAGATCCATGGTGCTGCCCAGA237TVEEVYQFSPFNEIHGAAQM781TGGAAGTAAAGCAATCTTTGGCTTTTGTTGAGATTGAGAAGACCCCTATTGCTCCATTTA257EVKQSLAFVEIEKTPIAPFK841AAGCTG

7、ATTACTTGGCTCGTGGATCTCTGCGGTATGAGTTTGCAAGTGAGATTCTTCAGG277ADYLARGSLRYEFASEILQA901CCCCCATTCAACTCATGAAGATCAGTGATGCACCAGCCCAGATTACTGAGATCCTGAAGC297PIQLMKISDAPAQITEILKH961ACTTGGTTGAAAACAATGTGGCCATGGTCCATGATGATGCTCCACTTAAGTTTGTTCAGC317LVENNVAMVHDDAPLKFVQL1

8、021TCGTCCAGCTCATGCGTGCTGCAACCTTTGAGGATATTGAGTCCATCTGGAAGCAGTGCA337VQLMRAATFEDIESIWKQCK1081AGGACAAACCAGTTTACAGGCGTTGGTTTCTGGATGCTCTTCCTGCTGTTGGTACACCAG357DKPVYRRWFLDALPAVGTPV1141TCATTGTAAAATTCATCAAGGAGAAGTTCCTGGATGGTGAACTTTCCATTCCTGAGTTCA377IVKFIKEKFL