enzymatic and whole cell catalysis finding new strategies for old processes

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1、BiotechnologyAdvances29(2011)75–83ContentslistsavailableatScienceDirectBiotechnologyAdvancesjournalhomepage:www.elsevier.com/locate/biotechadvResearchreviewpaperEnzymaticandwholecellcatalysis:FindingnewstrategiesforoldprocessesCarlaC.C.R.deCarvalho⁎IBB-Inst

2、ituteforBiotechnologyandBioengineering,CentreforBiologicalandChemicalEngineering,InstitutoSuperiorTécnico,Av.RoviscoPais,1049-001Lisboa,PortugalarticleinfoabstractArticlehistory:Theuseofenzymesandwholebacterialcellshasallowedtheproductionofaplethoraofcompou

3、ndsthathaveReceived14June2010beenusedforcenturiesinfoodsandbeverages.However,onlyrecentlywehavebeenabletomasterAccepted6September2010techniquesthatallowthedesignanddevelopmentofnewbiocatalystswithhighstabilityandproductivity.Availableonline15September2010Ra

4、tionalredesignanddirectedevolutionhaveleadtoengineeredenzymeswithnewcharacteristicswhilsttheunderstandingofadaptationmechanismsinbacterialcellshasallowedtheiruseundernewoperationalKeywords:conditions.Bacteriaabletothriveunderthemostextremeconditionshavealso

5、providednewandBiocatalysisBiotransformationextraordinarycatalyticprocesses.Inthisreview,thenewtoolsavailablefortheimprovementofbiocatalystsBioconversionarepresentedanddiscussed.Directedevolution©2010ElsevierInc.Allrightsreserved.BacterialadaptationKineticre

6、solutionContents1.Introduction...............................................................752.Improvingbiocatalyticactivity......................................................772.1.Isolatedenzymes........................................................

7、.772.2.Wholecells............................................................783.Novelenzymesproducedbyextremophiles................................................794.Productionofchiralcompoundsbybiocatalysis..............................................80

8、5.Concludingremarks...........................................................80Acknowledgement..............................................................80References................................