naoh预处理对秸秆高固体浓度酶水解效果影响

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1、中国沼气ChinaBiogas2015，33(3)17NaOH预处理对秸秆高固体浓度酶水解效果影响12333高静思，孙岩斌，文雪，王玮，刘研萍(1．深圳市清淼环保科技有限公司，广东深圳518055;2．中国城市建设研究院有限公司，北京100120;3．北京化工大学环境科学与工程系，北京100029)摘要:秸秆高固体条件下，酶水解有利于高浓度的还原糖产量，为了促进酶水解作用，探讨采用NaOH预处理对不同高底物浓度条件下酶水解的促进作用。结果表明:经过预处理后，酶水解还原糖总量最多增长了128%，酶水解得糖率也提高了约77%～114%，木质纤维素降解率也有较大幅度的提升，说明Na

2、OH预处理有利于酶水解的进行，并可以促进酶水解过程。综合考虑各因素，进行高固体酶水解实验适宜的底物固体浓度应选择15%。通过对未预处理、酶水解、预处理后酶水解的玉米秸秆的微观结构分析，可以看出，预处理并酶水解后玉米秸秆的结构发生了明显的变化，纤维束表面完全被破坏;红外谱图显示预处理并酶水解后玉米秸秆与糖类有关特征峰增多，为后续厌氧消化微生物提供了更易发酵的底物;X射线衍射图显示酶水解过程对无定形区的破坏作用较大，对结晶区的水解作用较小。NaOH预处理对酶水解过程有较明显的促进作用。关键词:预处理;酶水解;玉米秸秆;高固体浓度;还原糖中图分类号:TQ353;S216．4文献标志

3、码:A文章编号:1000－1166(2015)03－0017－06TheEffectofNaOHPretreatmentonEnzymaticHydrolysisofCornStrawwithHighSolidConcentration/GAO12333Jing-si，SUNYan-bin，WENXue，WANGWei，LIUYan-ping/(1．ShenzhenQingmiaoEnvironmentalTechnologyCo．Ltd，Shenzhen518055，China;2．ChinaUrbanConstructionDesign＆ＲesearchInstitute

4、COLTD，Beijing100120，China;3．DepartmentofEnvironmentalScienceandEngineering，BeijingUniversityofChemicalTechnology，Beijing100029，China)Abstract:Enzymatichydrolysiswasbeneficialtoleadtohighlevelsofsugarproductionunderhigh-solidconditionofcornstraw．Inordertopromoteenzymatichydrolysis，thepromoti

5、ngeffectofNaOHpretreatmentonenzymatichydrolysisofdif-ferenthighsolidsubstratewasstudied．Theresultsshowedthat，afterpretreatment，thetotalhydrolyzedsugarincreasedby128%maximally，theenzymatichydrolyticyieldincreasedby77%～114%，andlignocellulosedegradationrateimprovedtoo．ItindicatedthatNaOHpretre

6、atmentwasconduciveforenzymatichydrolysis，andcouldpromotethehydrolysisprocess．Consideringofvariousfactors，15%wasselectedasthepropersubstratesolidsconcentration．TheMicrostructureanalysis，andtheshowedthatthestructureofcornstrawhadsignificantchangesafterNaOHpretreatment，andthesurfaceofthefiberb

7、undlewascompletelydestroyed．TheFT-IＲshowedthat，aftertheNaOHpretreatmentandenzymatichydrolysis，thepeaksofcarbohydrateincreased，providingmorefermentablesubstrateforanaerobicdigestion．TheX-raydiffractionindica-tedthatenzymatichydrolysishadgreaterdestructive