棉花抗黄萎病鉴定与评价标准的商榷

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1、棉花学报CottonScience2017,29(1):50―58棉花抗黄萎病鉴定与评价标准的商榷*****赵丽红袁冯自力袁李志芳袁冯鸿杰袁师勇强袁张芸袁朱荷琴渊棉花生物学国家重点实验室/中国农业科学院棉花研究所袁河南安阳455000冤摘要院揖目的铱通过系统研究黄萎病抗病鉴定的关键技术和评价标准袁解决现有棉花品种黄萎病抗性评价标准中存在的问题袁建立新的田间棉花黄萎病分级标准和评价标准遥揖方法铱以14个2014年国家主推棉花品种为试验材料袁从接种量尧可靠样本量尧病情分级和评价方法4个方面系统研究黄萎病抗性鉴定的关键技术和评价标准遥揖结果铱确定了小区接种和行沟接种的最

2、佳接菌量分别为60g窑m－2和10g窑m－1袁小区调查最低样本量为50株曰制定了新的分级标准为0级渊0%冤尧1级渊1%～33%冤尧2级渊34%～66%冤尧3级渊67%～99%冤和4级渊100%冤遥连续2年利用相对病程发病面积和发病高峰期的相对病情指数2种评价方法判定品种抗病性袁前者作为抗病性评价的依据更为科学可靠袁一致率达到100%遥揖结论铱本试验为完善抗病鉴定技术操作规程袁规范抗性评价方法袁促进棉花抗病品种的选育尧审定和推广提供了科学依据遥关键词院黄萎病曰接种技术曰最低样本量曰分级标准曰抗性评价方法中图分类号院S435.621文献标志码院A文章编号院1002-7

3、807渊2017冤01-0050-09院10.11963/issn.1002-7807.201701006*****ZhaoLihong,FengZili,LiZhifang,FengHongjie,ShiYongqiang,ZhangYun,ZhuHeqin(455000,)[Objective]ThepurposeofthisinvestigationwastosystematicallystudythestandardtechniquesusedtoidentifyandevaluateVerticilliumwiltresistanceincottonto

4、solvethetraditionalmethodsordevelopanewone.[Method]Fourteennational-lyrecommendedcottonvarietiesin2014wereusedtosystematicallystudytheimportanceofinoculumconcentration,samplenumber,diseaselevel,andevaluationmethod.Wealsousedtherelativeareaunderthediseaseprogresscurveandtherelateddisea

5、seindexatthediseasepeaktoevaluateVerticilliumwiltresistanceincottonintwoconsecutiveyears.[Results]Theidealconcentrationsforplotandcolumninoculationswere60g窑m-2and10g窑m-1basedonthereal-timemonitoringofthediseasein-dexofsusceptiblevariety(Jimian11)plants.Toimprovethereliabilityofthedata

6、,theminimumnumberofinvestigatedplantswassetas50accordingtothedivergencerateanalysis.Thenewdiseasegradingstandardrelatedtocottonyieldwasbasedonthefoliardiseaserateandincludedthefollowinglevels:0level(0%),firstlevel(1%-33%),secondlevel(34%-66%),thirdlevel(67%-99%),andfourthlevel(100%).A

7、nddiseaseresistanceevaluationresultsintwoconsecutiveyearsshowedapplyingtherelativeareaunderthediseaseprogresscurveproduced100%consistentresultsbetweenyears.[Conclusion]Thepresentstudyprovidespotentiallyusefuldataforimprovingthetechniquesusedtoevaluatecottonvarietydiseaseresistance,and

8、mayst