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1、药物靶标的发现与验证技术研究进展论文【摘要】药物靶标的发现和验证是新药研发的第一步，也是药物筛选及药物定向合成成败的关键因素之一。随着基因组学、蛋白组学、生物信息学的快速发展，以及RNA干扰、噬菌体展示等新兴技术的出现，药物靶标的发现和验证技术日新月异。该文综述了在基因水平、转录水平、蛋白水平药物靶标发现和验证的各种技术的研究进展及其成功案例。【关键词】药物靶标;靶标发现;靶标验证Abstract：Drugtargetdiscoveryandverificationisnotonlythefirststepofinv
2、estigationofneostimportantfactorsindrugscreeninganddirectionalsynthesis.,MelendezAJ.DNAmicroarraytechnologyfortargetidentificationandvalidationJ.ClinExpPharmPhysiol,2006,33(5):371.3郑越,程肖蕊,周文霞,等.DNA微阵列技术在阿尔茨海默病及其防治药物研究中的应用J.生物技术通讯,2007,18(2):320.4GrunblattE,Mand
3、elS,MaorG,etal.GeneexpressionanalysisinN-methyl-4-phenyl-1,2,3,6-tetrahydropyridinemicemodelofParkinson′sdiseaseusingcDNAmicroarray:effectofR-apomor-PhineJ.JNeurochem,2001,78(1):1.5StamRL,MeijerinkJP,etal.DifferentialmRNAexpressionofAra-C-metabolizingenzymesexp
4、lainsAra-CsensitivityinMLLgene-rearrangedinfantacutelymphoblasticLeukemiaJ.Blood,2003,101(4):1270.6ThalMA,CarvalhoTL,HeT,etal.Ebf1-mediateddoiceJ.BiochemBiophysResmun,2009,378(4):851.8CaoRY,St.AmandT,Gr?bnerR,etal.Geicandpharmacologicalinhibitionofthe5-lipoxyge
5、nase/leukotrienepathentinApoEdeficientmiceJ.Atherosclerosis,2008,203(5):395.9SunZ,CadeR,ZhangZ,etal.Angiotensinogengeneknockoutdelaysandattenuatescold-inducedhypertensionJ.Hypertension,2003,41(2):322.10ChenolCellBiol,2009,29(11):3151.11RaoCV,LeiZM.Consequenceso
6、ftargetedinactivationofLHreceptorsJ.MolCellEndocrinol,2002,187(1):57.12JarvisMF,BurgardEC,McGaraughtyS,etal.A-317491,anovel,potentandselectivenon-nucleotideantagonistofP2X3andP2X2/3receptorsreduceschronicinflammatoryandneuropathicpaininratJ.ProcNatlAcadSciUSA,2
7、002,99(26):17179.13OkabeH,Furukaentanddifferentiationenhancingfactor-like1(DDEFL1)asadrugtargetforhepatocellularcarcinomasJ.IntJOncol,2004,24(1):43.14BrummelkampTR,BernardsR.NeammaliancancergeicsJ.NatRevCancer,2003,3(10):781.15HannonGJ,RossiJ.Unlockingthepotent
8、ialofthehumangenomemelkampTR,BernardsR,AgamiR.Stablesuppressionoftumorigenicitybyvirus-mediatedRNAinterferenceJ.CancerCell,2002,2(3):243.17LatterichM,AbramovitzM,Leyland-Jon
