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1、siRNA对人阴茎海绵体平滑肌细胞5型磷酸二酯酶表达的抑制作用siRNA?????L?b????????????????5?????????????????????S???d?F???xB???????B???????B??t????B???????B????3??Bn????B3B?????B??????o????RNA(siRNA)?????L?b????????????????5????????????(PDE5)?????????S???d,?x?L?b????????????(ED)??????????????????????BiB??
2、???B????d????Ambion????????????????????????PDE5??????siRNA???e,???)?n3??PDE5siRNA??1???L?p????siR2NA,???????L?b????????????????BiRT2PCR???Y????????siRNA??PDE5mRNA?????????S???d;Westernblot??????????????????????PDE5?????????L??BiB?????B?siRNA1BssiRNA2??siRNA3?????b???L?b??????
3、??????????PDE5mRNA?????5?u?E??58.2%Bs14.9%??11.9%;PDE5???????????E????70.5%Bs19.8%??17.3%;?L?p????siRNA???y?;??PDE5mRNA???????????????WBiB?????B????W?W???)?n??PDE5siRNA?????i?P?F?B?E?RPDE5????????,?m?,???e???i?p??siRNA?E?RPDE5???????????????m?,,?xED???????????????????.??BiBn?
4、??q??B3B?????????????;B?????????;B?????????;B?RNA????;B?PDE5????TheinhibitiveeffectofsiRNAonthephosphodiesterasetype5insmoothmusclecellsofhumancor2puscavernosumB?CHENGuo2qiang,BAIWen2jun,WANGXiao2feng,LIUShi2jun,HEPei2ying,HOUShu2kun.DepartmentofUrology,People9rsHospital,Peki
5、ngUniversity,Beijing100044,ChinaCorrespondingauthor:CHENGuo2qiangBnAbstractB3B?ObjectiveB?ToinvestigatetheinhibitiveeffectofsiRNA(smallinterferingRNA,siRNA)onthephosphodiesterasetype5(PDE5)insmoothmusclecellsofhumancorpuscavernosum,andprovideexperimentalgroundworkforthegeneth
6、erapyoferectiledysfunction(ED).B?MethodsB?SmallinterferingRNAstargetingPDE5geneweresysthesizedbyusingwebdesignsoftwareprovidedbyAmbion,theresiRNAsandcontrolsiRNAweresysthesizedbyAmbion.SiRNAsweretransfectedintosmoothmusclecellsofhumancorpuscavernosumbyusingsiPORTTMLipidreagen
7、t;down2regulationofPDE5mRNAwasdetectedbyRT2PCR;theinhibitiveeffectofPDE5wasdetectedbyWesternBlotting.B?ResultsB?TheresultsofRT2PCRin2dicatedsiRNA1BssiRNA2andsiRNA3madedown2regulationsofPDE5mRNAexpressioninthetransfectedgroups58.2%Bs14.9%and11.8%;thePDE5expressiondecreased70.5
8、%Bs19.8%and17.3%;howevertheexpressiondidnothavedifferentincontrolsiR
