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1、VascularendothelialgrowthfactorexpressingmesenchymalstemcellsimprovescardiacfunctioninchronicmyocardialinfarctioninpigsChinMedJ2006:119f19):1664—1668BriefreportVascularendothelialgrowthfaorexpressingmesen_.chymalstemcellsimprovescardiacfunctioninchronicmyocardialinfarctioninpigsYIFu,GUOWen-yi,L0A
2、n-lin,WANGHai-chang,LIHu,LIWei.jie,LIUBing,ZHANGDian.xin,LUANRong-hua,CHENGHe-xiang,LIFei,QINTao,ZHAOZhi-jing,GAOFengandJIAGuo.liangKeywords:myocardialinfarction;mesenchymalstemcelltransplantation;vascularendothelialgrowthfactor;ventricularfunctionTransplantationofmesenchymalstemcellsl(MSCs)formy
3、ocardiaIreconstructionhasshownpromiseinbothanimaImodelsandhumanphase1clinicaIstudies.1IVascularendotheliaIgrowthfactor(VEGF)isastrongtherapeuticagentfortreatingischaemiabyinducingangiogenesis.ThefeasibilityofexvivoMSCsmediatedgenetransferisdocumented.'.Matsumotoandcolleagues.haverecentlyreportedg
4、eneticallyengineeredMSCscarryingVEGF165deliveryforrevascuIarizatiOninamodelofacutemyocardialinfarctionfMI).ThepromisingdatafrOmourIaboratoryinbothangiogenesisandMSCstranspIantatiOnincunicularheartmodeIofacuteMIhavepromptedustoattemptthecombinedandsimultaneousapplicationofthetwostrategies.Inthepre
5、sentstudy,wecomparedtheeffec~oftransplantationofMSCs,transfectedwithorwithouttheVEGFgene,oncardiacfunctioninpigswithchronicmyocardialinfarction.METHODSAnimalsTheinvestigationconformedtotheGuidefortheCareandUseofLabora~ryAnimalspublishedbytheUSNationallnstitutesofHealthfNIHPublicationNo.85-23.revi
6、sed1996).Forthisstudy,thirtyhealthy,adultdomesticpigsofeithersex,weighingbetween50kgand65kgeach(ResearchCentreofLaboratoryAnimals,FourthMilitaryMedicaIUniversiChina)wereused.MSCsisolationandcultureBothdonomandrecipientsweredomesticpigsBonemarrowwastakenfrOmbreastbone.Bonemarrowmononuclearcellswer
7、eisolatedusingdensitygradientcentrifugationfNycoPrep1.077Animal;AXIS—SHIELDPoC,AS,Oslo,Norway).Then.themononuclearcellswereculturedinIowglucoseDulbecco'sModifiedEagleMediumcontaining10%foetaIbovineserumforMSCoutgrowth.