医学论文之 实验研究 (7)

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1、雷公藤多甙及雌孕激素治疗对大鼠卵巢Bcl2和Bax表达的实验研究作者：姜姣姣,郝丽,王瑞峰,黄婷,袁亮,徐星铭,卢文作者单位：安徽医科大学第一附属医院肾内科，合肥230022【摘要】目的探讨雷公藤多甙(TWP)致雌性大鼠生殖系统损害机制及防治方法。方法取50只SD雌性大鼠随机分为对照组,雌孕激素干预组(干预组),雌孕激素治疗组(治疗组),恢复组和雷公藤组。运用放免法测定血清性激素水平的变化,HE染色观察卵巢组织变化,免疫组化染色法检测卵巢组织颗粒细胞凋亡蛋白Bcl2和Bax的表达水平。结果与

2、对照组相比,雷公藤组和恢复组大鼠血清黄体生成素(LH)和卵泡刺激素(FSH)水平明显升高,雌激素(E2)的分泌明显下降,且差异有统计学意义(P值均<0.05);卵巢组织明显受损,各级卵泡明显减少,卵泡闭锁较多,干预组各指标则无明显改变;大鼠颗粒细胞凋亡蛋白Bcl2的表达在雷公藤组明显低于对照组,而干预组及治疗组均高于雷公藤组;Bax蛋白在各组的表达则相反(P<0.01),其中干预组与对照组无明显差异(P>0.05)。结论TWP可致雌性大鼠生殖系统的损害,雌孕激素替代治疗对卵巢功能有较好的保护作

3、用,其机制可能与Bcl2蛋白表达上调以及Bax表达下调有关。【关键词】雷公藤;生殖系统;激素替代EffectsofestrogenprogestogenreplacementtherapyandtripterygiumwilfordiipolyglycosidiumonexpressionsofBcl2andBaxinovarycellsofratsJIANGJiaojiao,HAOLi,WANGRuifeng,HUANGTing,YUANLiang,XUXingming,LUWen

4、(DepartmentofNephrology,FirstAffiliatedHospital,AnhuiMedicalUniversity,Hefei230022,China)【Abstract】ObjectiveToexploretheprotectiveeffectsofestrogenprogestogenreplacementtherapy(HPT)onimpairedgenitalsysteminducedbytripterygiumwilfordiipolyglycosidium

5、(TWP)infemaleratsanditsmechanism.Methods50SDfemaleratswererandomlydividedintofivegroups:controlgroup,interventiongroup,treatmentgroup,recoverygroupandTWPgroup.Thelevelsofserumgonadalhormoneweredetectedbyradioimmunoassay.Thehistologicalchangesoftheova

6、rywereobservedafterHEstaining.UsingtheimmunohistochemicalstainingtechniquewedetectedtheexpressionlevelsofapoptosisregulatoryproteinBcl2andBaxinovariangranularcell.ResultsThelevelofE2secretedbyovarywaslowerandthelevelsofLHandFSHsecretedbypituitarywer

7、ehigherinTWPgroupandrecoverygroupthanthoseincontrolgroup(P<0.05)andtherewerenosignificantdifferencesinlevelsofthesethreehormonesbetweencontrolgroupandinterventiongroup(P>0.05).InTWPandrecoverygroup,ovariantissuesweresignificantlyinjuredwithobviousdec

8、reasingofovarianfolliclesatdifferentstagesandmoreclausuraofthesefollicles.Comparedwithcontrolgroup,estrogenreplacementtherapycombinedwithprogestogencouldrestrainthesepathologicalchangesinducedbyTWP.InTWPgroup,Bcl2expressioninovarycellsofratswaslower