仙灵骨葆对兔膝骨性关节炎治疗作用的研究

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1、仙灵骨葆对兔膝骨性关节炎治疗作用的研究作者：程潭，张柳，李震，李强，穆树林，田发明，刘永强【摘要】[目的]研究仙灵骨葆(xianlinggubao,XLGB)对兔骨性关节炎关节软骨及软骨下骨的保护作用。[方法]30只3个月龄雄性日本大耳白兔，随机分为对照组(Sham)、盐水组(ACLT+NS)和仙灵骨葆组(ACLT+XLGB)，每组各10只。盐水组和仙灵骨葆组行右膝关节前交叉韧带切断术(anteriorcruciateligamenttransaction,ACLT)造成膝骨性关节炎模型。术后仙灵骨葆组125mg/(

2、kg·d)灌胃;盐水组则给予等剂量生理盐水。6周后处死动物，取股骨测量骨密度，之后脱钙制片，行HE染色、Mankin评分及BMP-2、MMP-13免疫组化染色;取右膝胫骨近端包埋，制成硬组织切片，用于骨组织形态计量学测量。[结果](1)Mankin评分：仙灵骨葆组显著低于盐水组。(2)股骨远端骨密度及胫骨近端软骨下骨骨量：仙灵骨葆组均显著高于盐水组。(3)免疫组化：仙灵骨葆组MMP-13的表达显著低于盐水组，BMP-2的表达显著高于盐水组。[结论]仙灵骨葆125mg/(kg·d)灌胃能够部分阻止骨关节炎兔关节软骨的退

3、变，其作用机制可能与下调MMP-13的表达，上调BMP-2的表达，同时增加软骨下骨骨量，改善其微观结构有关。【关键词】仙灵骨葆;骨性关节炎;软骨细胞;软骨下骨;BMP-2;MMP-1314　　Abstract:[Objective]ToassesstheprotectiveeffectsofXianlinggubaoonarticularcartilageandsubchondralboneinexperimentalosteoarthritisinducedbyanteriorcruciateligamenttra

4、nsaction(ACLT)inrabbits.[Methods]ThirtyhealthyJapanesewhiterabbitsweredividedrandomlyintothreegroups:shamgroup,ACLT+XLGBgroupandACLT+NSgroup.RabbitsinACLT+XLGBgroupandACLT+NSgroupreceivedanteriorcruciateligamenttransection(ACLT)ontherightknee.Rabbitsinshamgroup

5、receiveduntreatmentascontrol.ACLT+XLGBgroupreceivedadailyadministrationofXLGBatadoseof125mg/kgofbodyweightfor6weeks.Incontrast,ACLT+NSgroupreceivedsalineatthesamedose.Allrabbitsweresacrificedat6weeksafteroperation.Therightfemorawereremoved,measuredforboneminera

6、ldensity(BMD),andthenwereobservedforthemacro-pathologicchangeswithHEstainingandgradedbyMankin'sscale.ExpressionlevelsofBMP-2andMMP-13weredetectedbyimmunostaining.Thesubchondralboneofrighttibialplateauwascutandstainedforthemeasurementofbonehistomorphometry.[Resu

7、lts](1)TheMankin’sscoreinACLT+XLGBgroupwassignificantlylowerthanthatinACLT+NSgroup(P<0.05).(2)TheBMDofdistalfemoraandsubchondralbonemassofproximaltibiainACLT+XLGBgroupwassignificantly14higherthanthatinACLT+NSgroup(P<0.05).(3)InACLT+XLGBgroup:theexpression

8、levelofMMP-13wassignificantlylowerwhileBMP-2wassignificantlyhigherthanthatinACLT+NSgroup(P<0.05).[Conclusion]DailyadministrationofXLGBatadoseof125ug/kg/dfor6weekscanparti