effects of dietary β-glucan and glycyrrhizin on non-specific immunity and disease resistance of the sea cucumber （apostichopus japonicus selenka）challenged with vibrio splendidus

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1、J．OceanUniv．China(OceanicandCoastalSeaResearch)D0I10．1007／s1l802—010一l757—2ISSN1672—5182，20109(4)：389—394http：／／www．oucedu．cn／xbywb／E—mail．'xbywbOblC．educnImmunityandDiseaseResistanceoftheSeaCucumberusfiaoonitcusSelenkKa)ChnalnlenRedaCHANGJie)，ZHANGWenbing，MAIKangsen¨MAHon

2、gming¨andXUWei)，，，11TheKeyLaboratoO,ofMariculture，MinistryofEducationofChina，OceanUniversityofChina，Qingdao266003，尸R．China21SchoolofAnimalScienceandTechnology，InnerMongoliaUniversityfortheNationalities，Tongliao028000，R．China(ReceivedMarch31，201O：revisedMay18，2010；acceptedJ

3、uly1，2010)@OceanUniversityofChina．SciencePressandSpringer—VerlagBerlinHeidelberg2010AbstractSeacucumbersApostichopus]aponicusSelenka，werefeddietscontainingnon—immunostimulant(basaldiet)，0．2％13-glucanand0．02％glycyrrhizininarecirculatorywatersystemfor45days，andsubsequentlych

4、allengedwithVibriosplendidusbyinectionat1．0×10cfu／seacucumberforl5days．Phagocyticcapacity(PC)，intracellularsuperoxideanionproduction(ISAP)，ly—sozyme(LSZ1activityandsuperoxidedismutasefSOD)activityinthecoelomicfluidwereanalyzedonthe0，5，l0“and15“daysafteriniection．Resultssho

5、wedthatafterthe45一dayfeedingperiod，PC，ISALSZactivityandSODactivityinseacucumbersfedwithdietary3-glucanorglycyrrhizinweresignificantlyhigherthaninthosefedwiththebasa1diet．Onthe5dayafterinfection．alltheimmuneparametersexaminedintheseacucumbersinjectedwithsplendidusdecreasedi

6、nvaluesignificantly．Onthe15“day，PC．ISAPandLSZactivityreturnedto1evelssimilartothoseonthe0“day．Fortheseacucumbersinjectedwithsaline，therewerenosignificantdifferencesinalltheimmuneparametersexaminedandinthecumulativemorbidityduringthe15-daychallengingtria1Afterinjectingwiths

7、plendidus．thecumulativemorbidityofseacucumbersfedwiththebasaldietwassignificantlyhigherthanthosefedwithdietary3-glucanorglycyrrhizinwhenchallengedwithsplendiduschallengedseacucumberfedwiththebasaldietwassignificantlyhigherthanthosefedwithdietary3-glucanorglycyrrhizin．There

8、wasnosignificantdifferenceincumulativemot—biditybetweenthedietary3-glucanandglycyrrhizint