各类藻类培养基

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1、AJS(AcidifiedJM:SE)FreshwateralgaDunaliellaacidophilaMediumAcidified97:3mixtureofJMandSE2Seeseparaterecipes(JMandSE2).Forapproximately1 litreoffinalmedium,mix970 mlJMwith30 mlSE2.Add10 mlofconcH2SO4togiveapHofapproximately1.5.Autoclaveat15psifor15minutes.ANT(Antia'sMedium)MarinealgaeStockTracemeta

2、lsstocksolution(chelated)perlitreEDTANa2.2H2O3.24gFeCl3.4H2O1.08gMnSO4.4H2O0.450gZnSO4.7H2O0.230gNa2MoO4.2H2O0.097gCuSO4.5H2O0.01gCoSO4.7H2O0.0056gMakeupto1litrewithdistilledwaterandadjustpHto7.6-7.8withdiluteHClorNaOH.Storefrozen.MediumperlitreKNO30.05gNaH2PO4.2H2O0.0078gTris[tris(hydroxymethyl)a

3、minomethane]1.0gGlycine0.3gTracemetalsstocksolution(chelated)2.5mlThiamineHCl500.0µgCyanocobalamin2.0µgBiotin1.0µgFilterednaturalseawater800.0mlMakeupto1litrewithdistilledwaterandautoclaveat15psi.FinalpHshouldbe7.6-7.8.RefAntia,Cheng&Taylor(1969)ASW(ArtificialSeawater)MarinealgaeStocks(1)Extrasalt

4、sperlitreNaNO330.0g Na2HPO41.2gK2HPO41.0g(2)VitaminsolutionperlitreBiotin0.0002gCalciumpantothenate0.02gCyanocobalamin0.004gFolicacid0.0004gInositol1.0gNicotinicacid0.02gThiamineHCl0.1gThymine0.6gMaybestoredfrozenat-20°CMediumperlitre*"UltramarineSynthetica"seasalts33.6gExtrasaltsstocksolution(1)3

5、.75mlVitaminstocksolution(2)2.5mlSoilextract(SE1-seerecipe)25.0mlTricine0.50gMakeupto1litrewithdistilledwaterandadjustpHto7.6-7.8with1MNaOHorHCl.Autoclaveat15psi.Supply*WaterlifeResearchIndustriesLtd,476BathRoad,Longford,WestDrayton,MiddlesexUB70ED,UK.Tel(01753)685696Fax(01753)685437ASW+barley(Art

6、ificialSeawater+barleygrains)MarinedinoflagellateOxyrrhisMediumFollowtherecipeforASWbutaddapproximatelyonegrainofbarleytoeach25mlofpreparedmediumpriortoautoclaving.2ASW(doublestrengthArtificialSeawater)MarinealgaeMediumFollowtherecipeforASWbutadd35gNaClperlitrepriortoautoclaving.ASW:BGMarinealgaeM

7、edium1:1mixtureSeeseparaterecipes.Mixaseptically.ASW:SESMarinealgaeMedium3:1mixtureSeeseparaterecipes.Mixbeforeautoclaving.ASWP(ArtificialSeawaterforProtozoa)MarineprotozoaStockSolution(1)perlitreNaNO35.625gNa2HP