功能化多肽量子点的制备及其生物医学应用

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1、硕士学位论文fluorescenceandcolloidalstabilityofthequantumdotsindifferentpH，saltconcentration，cellimagingandcelltoxicityteststoevaluatethequantumdots．Besidesquantumdotswhichcontainingcadmium，non-toxicZn-basedandAg-basedquantumdotshavebeenresearchedandcomparedwithCdcontainingQDsonthein

2、fluenceofcellactivity,tobebetterinbiologicalapplications．Chapter1．AqueousphasesynthesisandpropertiesofCdTequantumdotsThissectionmainlystudiedtheaqueousphasesynthesisofCdTequantumdots．WccarriedoutaqueousphasesynthesisofCdTequantumdotswitllhydrophiliccoatingsof1-thioglycerol，thio

3、glycolicacid，mercaptopropionicacid，L-cysteineandacetylcysteine，respectively．InfluenceoftheCd／Temole，reactiontimeanddifferentcoatingsontheopticalpropertiesofCdTcquantumdotswereinvestigated．Throughcomparingtheopticalpropertiesbyabsorption，emissionwavelength，thefluorescencequantum

4、yieldandthefullwidthhalfmaximum，wefoundthatQDwithcoatingsof1-thioglycerolslowestgrowthandthioglycolicacidfastest-growing．CdTcquantumdotswithcoatingsof1-thioglycerolhavethenarrowestFWHMandwellmonodisperse，higherquantumyieldcanalsobeseenwhencoatingsofacetylcysteine．ChangetheCd，Te

5、molarratio，wefoundthatCd／Teratioof1：1．23generatedquantumdotsFWHMisquitenarrowandhighquantumyield．Moreover,thefluorescentmicroscopyimagesshowedthehydroxylgrouponTGCanbcusedtominimizenonspecificcellularbindingofQDs．Chapter2．One-stepsynthesisofpeptide—programmedQDsforbiomedicalapp

6、licationThissectiononthebasisofthefirstportionundertheoptimalreactionconditionstobctested．Majorintroducethemethodofone-stepsynthesisofpeptide-programmedQDsinaqueousphase．AtypicalsynthesisofCdTeQDsisdescribedasfollows：First，sodiumhydrogentelluride(NaI-ITe)Wasfreshlymadebeforeeac

7、hsynthesisbydissolving0．025gsodiumborohydride吖aBH4)in1“deionizedwaterandthen0．040gtelluriumpowderWasaddedintotheNaBH4solution．ThisreactionWasconductedatroomtemperatureovernightinaneppendorftube．CdCl2．2．5H20(14．5rag)and12I_tl1-thioglycerol(1．259／m1)weredissolvedinABSTRACT50mlult

8、rapurewaterandstirredfor10min．Thesolutionwasadjustedto