高产纤维素酶菌株选育、分子鉴定及发酵工艺研究

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1、中南林业科技大学硕士论文高产纤维素酶菌株选育、分子鉴定以及发酵工艺条件研究ABSTRACTCellulasehasgreatapplicationvalueinalmostallplantsfortheprocessingofrawmaterialsSuchasbrewing，fi'uitandvegetablejuiceprocessing，foodprocessing，food，feed，paperandextractionofeffectivecomponentsofChineseherbalmedicine，andplantingeneticengineering，cell

2、engineering，oilextraction，textileindustry,wastewatertreatment，bio-energyproduction，etc．Ithasmanyresearchpapersaboutcellulose，buttheseriousproblemwasthestrainsushasTrichodermareesei，Penicilliumwhichproducedcellulosehadlowertheactivityofcellulase．Sotheamoutofthecellulasewerelagerly,whichcausedhi

3、ghcost．High—yieldcellulaseproducingstrain，theoptimumconditionsoffermentationandsuitableconditionofenzymereactionsystemcaneffectivelyreducecellulaseexpenses．Inthisstudy,anexcellentcellulase-producingstrainWasscreenedfromtheforestfloorunderrottingwoodandsoilinYueluMountain．Itwasmutatedsequential

4、lybydifferentphysicalmethods，andmicroscopicobservationand16srDNAsequencealignmentwereusedforidentificationmutantstrain，besides，thefermentationconditionandtheconditionsenzymereactionsystemandenzymaticpropertieswerestudied．Themainresultswereasfol!ows：Anexcellentcellulase—producingstrainA24wasscr

5、eenedfromtheforestfloorunderrottingwoodandsoilinnature，andtheactivityofCMCaseandFPAasewere20．37U／mL，1．20U／mL，respectively．TheoriginalstrainA24WastreatedwithUVmutagenesis，andthebestUVmutationtimewas7min．Inthiscondition，amutantstrainUV-37hadgoodgeneticstabilityandtheactivityofCMCaseandFPAasewere

6、25．35U／mLand1．33U／mL，respectivly．Thebest60Co吖raymutationdosewas0．6KGyforthemutantstrainUV-37．Inthiscondition，amutantstrainCo-07whohadgoodgeneticstabilityandtheactivityofCMCaseandFPAasewerehigherthantheoriginalstrain137．16％and118．80％．Themorphologicalcharacteristicsofcolonyandmyceliumandsporewir

7、ewereobservedbyMicroscope．Frommicroscopicobservation，radialcolony,substratemyceliumandaerialhyphahelicinesporewirewereobtained，anditcouldbeStreptomyces．16srDNAwasamplifiedandsequenced，andthenNCBIdatabaseWas中南林业科技大学硕士论文高产纤维素酶菌株选育、分子鉴定以及发