草菇半纤维素相关酶活性及其基因表达研究

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1、万方数据ABSTRACTRESEARCHONTHEENZYMESACTIVITIESANDGENEEXPRESSIONOFHEMICELLULASE烈M睨谢尺腰L叫舰黝C别Volvariellavolvaceaisoneofimportantgrass—rotfungiwhichgrowintheenvironmentofhighhumidityandtemperature．Volvariellavolvaceausesthecropstrawrichedincelluloseandhemicellulaseas

2、thegrowthsubstrate．StrawmushroomCansecretehemicellulasetoprovidethenutrientsneededforgrowththroughdegradatinghemicellulose．ButVolvariellavolvaceashowsalowbiologicaltransformationefficiencywimonly10—40％comparedtootherediblemushrooms，suchasAgaricusbisporusshows

3、biologicalefficiencyupto80-100％．ThehemicellulosedegradationmechanismofVolvariellavolvaceaisverycomplex．Westudythehemicellulasegenesexpressionandcellulaseactivityinstrawmushroom，andtrytofindtherelationshipamongthehemicellulosedegradingenzymegene，enzymeactivity

4、andbiologicaltransformationefficiency．TheresultsmayhelptoimprovethebiologicaltransformationefficiencyofVolvariellavolvaceaandhavebenefitsforstrawmushroomindustry．Inpreviousstudies，thewhole—genomesequencingprojectofVolvariellavolvaceahadbeencompleted，andthegen

5、ome—wideframeworkmaphadbeenestablished．Basedontheseresults，wefindoutthegeneencodinghemicellulasedegradingenzymesbybioinformaticsanalysis．Hemicellulasedegradingenzymesstructurebetweengrass—rottingfungiandwood—rottingfungiwerecomparedalldtrytoexplorethereasonsf

6、orthelowbiologicaltransformationefficiency．1．Thereare17strawmushroomstrainsusedforfruitingexperiment．Throughcalculatingthebiologicaltransformationefficiency,17strainsweredividedinto9categoriesthroughsignificantanalysis：First，Pinyouyihao；Second，Vn一1，Z—VH3；Thir

7、d，Vn一3，0229，V23；Fourth，VH3，TaiguoNO．1，WGVbl；Fifth，Z—VH3，Dagutu034，Tu031，AS；Sixth，SiliancaoguNo．4，427—434；Seventh，3560．Therearenosignificantdifferencesofbiologicaltransformationefficiencyininternalstrainsofeachtype，buttherearesignificantdifferencesbetweenclass

8、es．ThestrainPinyouyihaohasthehighestbiologicalefficiencyof21．73％，while3560hasthelowestbiologicalefficiencyof1．95％．Threestrainsofhigh，mediumandlowbiologicaltransformationefficiencyaresampl