大气pm2.5气管滴注染毒对大鼠心肺毒性的研究

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1、授予单位代码10089学号或申请号20152251中国图书分类号R134HebeiMedicalUniversity硕士学位论文学术学位大气PM2.5气管滴注染毒对大鼠心肺毒性的研究研究生：胡金凤导师：牛玉杰教授张荣教授专业：劳动卫生与环境卫生学二级学院：公共卫生学院2018年3月目录中文摘要···········································································································1

2、英文摘要···········································································································3英文缩写···········································································································6研究论文大气PM2.5气管滴注染毒对大鼠心肺毒性的研究前言··

3、·········································································································8材料与方法·······························································································9结果··············································

4、···························································19附图·········································································································23附表···················································································

5、······················36讨论·········································································································41结论·········································································································44参考文献·········

6、························································································44综述PM2.5短期毒性研究进展·····································································51致谢·············································································

7、···································65个人简历·········································································································66中文摘要大气PM2.5气管滴注染毒对大鼠心肺毒性的研究摘要目的：通过气管滴注染毒，探讨28天重复暴露PM2.5对大鼠心肺的毒性作用。方法：1PM2.5的采集、制备和表征大流量颗粒物采样器采样、聚四氟乙烯（PTFE）

8、采样膜收集PM2.5；超声浸提-冷冻干燥法制备PM2.5样品。透射电子显微镜（TEM）观察PM2.5尺寸和形态；纳米粒度及Zeta电位分析仪分别测量PM2.5的粒径分布和Zeta电位。2动物模型的建立32只SD大鼠随机分为4组；对照组和三个剂量组5mg/kg、10mg/kg和20mg/kg；气管滴注法暴露PM2.5，对照组气管滴注相同体积对照提取液，每周染毒2次，间隔2天，连续4周，最后一次滴注后24小时处死。3大鼠肺组织损伤