资源描述：
《植物生物技术概要1》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、Anauxinresponsive CLE generegulatesshootapicalmeristemdevelopmentin ArabidopsisPlanthormoneauxinregulatesmost,ifnotallaspectsofplantgrowthanddevelopment,includinglateralrootformation,organpattering,apicaldominance,andtropisms.Peptidehormonesarepeptidesw
2、ithhormoneactivities.Someofthefunctionsofpeptidehormonesinregulatingplantgrowthanddevelopmentaresimilartothatofauxin,however,therelationshipbetweenauxinandpeptidehormonesremainslargelyunknown.Herewereporttheidentificationof OsCLE48,arice(Oryzasativa) CL
3、E (CLAVATA3/ENDOSPERMSURROUNDINGREGION)gene,asanauxinresponsegene,andthefunctionalcharacterizationof OsCLE48 in Arabidopsis andrice. OsCLE48encodesaCLEpeptidehormonethatissimilarto Arabidopsis CLEs.RT-PCRanalysisshowedthatOsCLE48 wasinducedbyexogenously
4、applicationofIAA(indole-3-aceticacid),anaturallyoccurredauxin.Expressionofintegrated OsCLE48p:GUS reportergeneintransgenic Arabidopsis plantswasalsoinducedbyexogenouslyIAAtreatment.Theseresultsindicatethat OsCLE48 isanauxinresponsivegene.Histochemicalst
5、ainingshowedthat GUS activitywasdetectedinallthetissueandorgansofthe OsCLE48p:GUS transgenic Arabidopsis plants.Expressionof OsCLE48 underthecontrolofthe 35S promoterin Arabidopsis inhibitedshootapicalmeristemdevelopment.Expressionof OsCLE48 underthecon
6、trolofthe CLV3 nativeregulatoryelementsalmostcompletelycomplemented clv3-2 mutantphenotypes,suggestingthatOsCLE48isfunctionallysimilartoCLV3.Ontheotherhand,expressionof OsCLE48 underthecontrolofthe 35S promoterin Arabidopsis haslittle,ifanyeffectsonroot
7、apicalmeristemdevelopment,andtransgenicriceplantsoverexpressingOsCLE48 aremorphologicallyindistinguishablefromwildtypeplants,suggestingthatthefunctionsofsomeCLEpeptidesmaynotbefullyconservedin Arabidopsis andrice.Takentogether,ourresultsshowedthat OsCLE
8、48 isanauxinresponsivepeptidehormonegene,anditregulatesshootapicalmeristemdevelopmentwhenexpressedin Arabidopsis.IntroductionAuxinregulatesmanyaspectsofplantgrowthanddevelopment,includingapicaldominance,organformation,lateralroot
