针刺对脑缺血大鼠神经元凋亡的影响论文

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1、针刺对脑缺血大鼠神经元凋亡的影响论文【摘要】目的观察针刺对大鼠局灶性脑缺血再灌注损伤后海马神经元内细胞周期蛋白依赖性蛋白激酶4(CDK4)和神经元凋亡的影响。方法大脑中动脉线栓法建立脑缺血再灌注损伤模型，再灌后针刺大鼠，应用免疫组化、免疫印迹法和流式细胞计数法分别检测CDK4和细胞凋亡。结果缺血组再灌注48h后海马CDK4表达升高，凋亡细胞增多，针刺组CDK4表达和凋亡细胞明显减少(P0.01)。结论针刺对脑缺血再灌注损伤有保护作用，其机制可能与调控细胞周期因子从而抑制凋亡有关。【关键词】针刺;脑缺血;细胞周期;凋亡ABSTRACT：Obj

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