川芎嗪对大鼠急性脊髓损伤模型il-10 il-18表达的影响

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1、硕士学位论文中文摘要统计学意义。术后1小时脊髓前角IL一18表达阳性细胞计数即表达升高，术后24小时即达最高，；术后12小时，24小时、3天、7天和14天5个时间点上，实验组IL—18表达数低于对照组，P<0．05，有统计学意义。改良Rivlin斜板实验，术后斜板临界度数均值逐渐升高，实验组较对照组斜板临界度数高，术后7天及14天时，两组间斜板临界度数比较t检验P<0．05，有统计学差异。BBB评分，术后BBB评分均值逐渐升高，实验组较对照组评分值高，术后7天和14天时，实验组和对照组有统计学差异(P

2、嗪对大鼠脊髓继发性损伤有保护作用：川芎嗪能够促进大鼠急性脊髓损伤后IL．10的表达、抑制IL．18的表达。关键词：脊髓损伤(SCI)，J11-墨5嗪(TMP)，白细胞介素10(IL-10)，白细胞介素18(IL-18)硕士学位论文英文摘要ABSTRACTOBJECTIVE：TostudytheeffectofTeramethylpyrazine(TMP)onexpressionofIL--10andIL·-18inacutespinalcordinjury(ASCI)inratsandapproachtheprotectiv

3、eeffectandpossiblemechanismofTMPinASCI．METHODS：88SDratsweighting2509upto3009weredividedrandomlyintothreegroups：(1)Shamoperationgroup(groupS，n_8)；(2)Controlgroup(groupC，n=40)；(3)Tramethylpyrazinegroup(groupT’n=40)‘．ThemodifiedAllen’SmodelofASCIatT10sectionWasestabli

4、shed．200mg／kgofTMPwasusedtotheTgroupintraperitoneallyandtheSandCgroupsweretreatedwiththesamevolumeofsalinevehicle(NS)30minutesaRerinjury．Eachmodelwasgiventhes御'nedosageonceadayuntilsacrificeorlastedfor5daysfollowingtheinjuryiftheratssurvivedformorethan5days．Therats

5、ofthreegroupswerekilledtocollectthespinalcordtissuesamplesfromtheinjuredsegmentwiththelengthof1cmattheeachtimepointfollowingtheinjury．Eachsamplegothistologicalsectionsof10umthicknessthroughmicrotomyinthemiddleofthesample．ThesectionwaspreparedtotesttheIL-10andIL-18b

6、yimmunohistochemistrymethodseparately．ThenumberoflLsineverysectionwascounted，andtheaveragepositiveILscellsofeachgroupatthesametimepointwerecalculated．SoftwareSPSS13．0Wasappliedtoanalysisiftherewassignificantdifferenceamongthethreerelatedgroupsateachtimepoint．Animal

7、behavioralscores———modifiedRivilinloxoticplateandBBBscoreonspinalcordfunctionaldeficits硕士学位论文英文摘要wereperformedtotheratssacrificedatthe24hour，3day，7dayand14dayfollowingSCI，andanalysisthedifferencebetweenthegroupCand，ngroup1．RESULTS：TheoutcomesofratsinmodifiedRivlinl

8、oxoticplatetest：TgroupcomparedwithCgroupat7d，14dafterSCIrespectively(p