克雷伯氏杆菌的甘油代谢振荡机理及发酵过程在线监测-生物化工专业毕业论文

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1、克雷伯氏杆菌的甘油代谢振荡机理及发酵过程在线监测StudyontheoscillationmechanismandonlinemonitoringofglycerolmetabolismbyKlebsiellapeneumoniaeAbstractAsthemostimportantchemicalmaterial，1,3-propanediolhaswidespreaduseinmultipleareasincludingcosmetic．medicineetc．Itattractconsiderableinterestespeciallyforitca

2、nsynthesispolytrimethyleneterephthalate(PTT)asthemonomer．Comparedtotraditionalchemicalsynthesismethods．thebiologicalmethodsiSmoreenvironment—friendlyandfulfillsthesustainabledevelopment．Usinghighconcentrationofsubstrateduringcontinuousfermentationisaneffectivemethodtoenhancethecon

3、versionofsubstrate，productconcentrationandyield．However，withthehighconcentrationofsubstrateorproductsorbothofthem1cadtooscillationorpolymorphicetc．ThisthesisiSaimedtodiscussthemainreasoninducetheoscillationduringcontinuousfermentationofglycerolbyK．peneumoniae．Firstly，westudyontheo

4、scillatorybehaviorofKpeneumoniaeincontinuouscultureinducedbyhighconcentrationofglycer01．Theglycerolwasenhancedfrom20∥Lto100edLleadtoinhibitthegrowthofcell，thestateoffermentationshiftintolagphase．Rightnow．theconcentrationofthetoxicmiddlemetabolites，3-hydropropionaldehyde(3-HPA)hadc

5、limbedto0．63g／Lthenfellto0．18∥L．Othermetabolitessuchas1，3-propanediol，aceticacidalsoreachediI'Slowestpoint，after20hthehighconcentrationofglycerolofadaptation．ThemetabolitesincludingtheC02，1，3．propanediol，aceticacid，3-HPA，formicacid，succinicacidetcstartstooscillate．Themainoscillati

6、onperiodof6．5．7．5h，andtheoscillationstateCan1astformorethanl40h．Besides，3-HPAhasashorteroscillationperiodof3．4h．thechangeintheconcentrationofitduringtheoscillationstatearefrom0．13．O．78∥L．Comparedthemetabolicfluxoftheoscillationstateandthestablestateshowsahigherproductivityandspeci

7、ficproductionrateofmetabolitesCanbeachievedduringtheoscillationstate，andtherehasnoaccumulationoftoxicmetabolite3-HPA．Soweanalyzetheoscillationfromthe1evelofmetabolitesinducedbyhighconcentrationofglycerolindicatethat3．HPAmightbethemainreasonforthis．Secondly．transcriptomeanalysesuse

8、dtostudytheoscillatorybehavior．In