水产品中副溶血性弧菌的elisa快速检测

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1、上海水产大学硕士论文PAbto坳islxl04cfu／mL；ThecrossingtestandinhibitiontestshowsthatthePAbisstronglyspecial．Sothisworkwilllayafoundationforlaterwork．2EstablishmentofIndirectELISARapidMethodInthispaper,theoptimumreactionconditionofeverydetectionprocedurewasdeterminedt

2、hroughfiltrationexperiment，andtheoptimumworkconcentrationoftheAgandantibodybyusingcheckerboardtitration．Afterthemethodwasstandardized，thedetectionparameterswerebelow：Usingthenewcoatingmethod(dryingcoatingat60℃1；ThecoatingconcentrationofAgis107cfu／ml；Clos

3、ingtimeis2．5h；Theworkdilutionoffirstantibodyis1：4000；TheincubationtimeofAgreactingwithfirstantibodyis75min；Thedilutionofthesecondaryantibodylabeledenzymeisl：1000；Theincubationtimeoffirstantibodyreactingwiththesecondaryantibodylabeledenzymeis60min；Thereac

4、tiontemperatureofenzymeandsubstrateis30"C，timeis15min；theincubationtemperatureis3TC；UsingthemethodtodetectthebacteriumsuspensionofEp，thedetectionlimitandtimeare104cfu／mland8hseparately．3IndirectELISARapidMethodforDetecting印TheindirectELISArapidmethodford

5、etecting印WasestablishedbyusingtheantibodyastheIn'stantibody,andthegoatanti—rabbitlgG-HRP髂thesecondaryantibodylabeledenzymeseparately．ThenthestandardizedmethodWasusedfordetectingtest．ThecrossingtestresultshowsthatthecrossreactionbetweenthePAbandotherbacte

6、riaisnegative．TheinhibitiontestshowsthatthePAbisspecialstrongly,andtheinhibitionratioreaches86．53％．Usingthismethodtodetectseafoodinfectedwithbacteriaartificiallyandactualseafoodrespectively,thelowestdetectionlimitis104cfu／ml，thedetectiontimeis8h；However,

7、thedetectionlowerlimitWasincreasedto10’cfu／mlbyculturingthebacteria．Thismethodisconsistentwiththeconventionalmethodtestresultcompletely．Therefore，itCallofferanexact，rapidmethodfordetecting脚inseafood，andalsohasveryhighpracticalapplicationValRe．4Developmen

8、tofindirectELISAdiagnostickitforrapiddetecting吻inseafoodThecomponents，userintroduction,operationnoticesofindirectELISAdiagnostickitforrapiddetecting印inseafoodweregiven．Thenwecarryouttheseexperimentaboutstoragelifeofthekit’