plga-ha-pva支架复合兔软骨细胞的动物体内实验-研究

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1、万方数据InvivostudyPLGA-·HA--PVAscaffoldrabbitchondrocytesAbstractObjective：ComparisonofdifferentratioofPVAcompositescaffoldsodorinducedrabbitcartilageinvitroandinvivointocartilage．Method：(1)bycollagenaseenzymedigestionandmechanicalseparationCansuccessfullygettherabbitchondrocytes：(2)inthecaseofwi

2、thoutinducingfactor，PLGA-HA—PVAcompositescaffoldscanbeusedascellscaffoldpromotecartilagecellproliferation，PVA，theratioof15wt％，5wt％HA，30wt％ofPLGAcompositescaffoldforcellscaffold；(3)Inthecaseofnoaddedinducingfactor，PLGA—HA-PVAcompositescaffold／rabbitchondrocytescomplexescanectopicproliferationin

3、mouseskin，wheretheratioof15wt％PVA，5wt％HA，30wt％PLGAincompositescaffoldbracketbetter．Results：(1)bycollagenaseenzymedigestionandmechanicalseparationcansuccessfullygettherabbitchondrocytes；(2)inthecaseofwithoutinducingfactor，PLGA—HA—PVAcompositescaffoldscanbeusedascellscaffoldpromotecartilagecellp

4、roliferation，PVA，theratioof15wt％，5wt％HA．30wt％ofPLGAcompositescaffoldforcellscaffold；Conclusions：WithouttheadditionofinducingfactorsPLGA-HA-PVAcompositescaffoldsinvitroandinvivotoadvanceectopicrabbitchondrocyteproliferation，inwhichtheratioof15wt％PVA，5wt％HA，theeffectof3Ovvt％PI，GAscaffoldsfortheb

5、etter．Keywords：Tissueengineering；scaffolds；Cartilagedefect．PLGA；HA，PVAClassification：Ibl8．08III万方数据目录学位论文原创性声明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯I摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．IIAbs仃act⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯III目录⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

6、⋯⋯⋯．．IV1．绪{仑⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．12．材料与实验方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯32．1主要的实验试剂和仪器⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯32．2支架材料的制备⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯52_3软骨细胞的分离、培养与鉴定⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯62．4PLGA．HA．PVA复合水凝胶与软骨细胞复合体外培养⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．72．5PLGA．HA．PVA与软骨细

7、胞复合体植入裸鼠背部皮下⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．82．6实验标本的处理⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯82．7实验标本的检测方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯92．8实验数据的处理⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．1l3．结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．。123．1复合支架的制备⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯