apc和eb1基因表达与染色体数目异常胚胎关系

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1、重庆医科大学硕士研究生学位论文T唧RELAnoNSⅢPoFAPCANDEBlGENEEXPRESIoNSWITHCHRoMoSoM哐NUM皿ERBackground：ABSTRACTTherateofchromosomenumberabnormityisabout3％inpopulation，butinspontaneousabortion，itisroughly30％．Mostofchromosomenumberabnormityembryosshowcelldivisionrestrained，e

2、vencauseembryotodie．Theattachmentbetweenthespindlesandkinetochoresprovidesessentialfunctionsforaccuratechromosomesegregation．Withthetractionofspindles，chromosomesgraduallymovetothepolesofcellsanddistributeevenlytodaughtercells．Butbynow,themolecularbasis

3、forchromosomenumberabnormityisunknown．Itwasreportedthatchromosomenumberabnormitymaybedue，atleastinpart，tomutationsintheadenomatosispolyposiscoli(APC)inacoupleofpapers．APCasatumorinhibitingfactorhasbeenwellinvestigatedinoncology．Buttheseinvestigationsmos

4、tlyfocusedon、ⅣNTsignalpathwayandfoundAPCCannegativelyregulateB—catenin，whichisaimportantcomponentofthe、7ⅢTsignalpathway．However,besidesofnegativelyregulateintheWNTsignalpathway,APCCanalsoregulatemicrotubuleassembling，stability5重庆医科大学硕士研究生学位论文andattachme

5、ntofspindlestokinetochores．Moreover，theresearchesODthestructureofAPCindicatedthatthereisastructuraldomainintheC—terminalofAPCthatcarlbindwithEnd—bindingproteinI(EB1、．Withthemanner,APCandEB1takepartinorregulatetheinteractionsofspindlesandkinetochorestoge

6、ther．EB1istheoneofmicrotubuleplus—terminal-bindingproteins，andcancombinewithmicrotubules’plus—terminalsolely．Bythismeans，EB1canmakemicrotubulesgrowtowardscentromeresandstabilizemicrotubules．Furthermore，EB1canregulatemicrotubulesbindingwithCLIPsthatarean

7、othergroupmicrotubuleend—bindingproteinsandinteractionofAPCandB—catenin．TheseinvestigationsindicatedAPCandEB1takeimportantroleintheinteractionofspindlesandcentromeres，andinmanytypesofAPCmutationtumorcellsandthecellstrainswhichAPCandEB1overexpressedbyman

8、—madeappearedabnormalcelldivisions．Asweknow,fetationandoncogenesisbehavesimilarcharacterinmanyfacets．So，itisasignificantstudytoinvestigateifAPCandEB1takecrucialroleinchromosomesabnormalembryoniccellsObjective：ToinvestigateAPCandE