资源描述：
《类人胶原蛋白-透明质酸血管支架的性能及生物相容性》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、生物工程学报ChinJBiotech2009,April25;25(4):591-598journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn©2009InstituteofMicrobiology,CAS&CSM,Allrightsreserved组织工程与细胞培养类人胶原蛋白-透明质酸血管支架的性能及生物相容性1,31,21,22232孙秀娟,范代娣,朱晨辉,马晓轩,骆艳娥,陈岚,郭佳庆1西北大学陕西省可降解生物医药材料重点实验室,西安7100692陕西省生物材料与发酵工程
2、技术研究中心,西安7100693西北大学生命科学院,西安710069摘要:将类人胶原蛋白与透明质酸按不同比例复合,控制透明质酸的终浓度(W/V)分别为0、0.01%、0.05%、0.1%,用京尼平交联,采用真空冷冻干燥方法构建出血管支架材料。通过扫描电镜、XPS分析、拉力测试、压力爆破实验、细胞毒性实验、血管支架细胞种植实验及小鼠皮下植入等方法对其表面超微结构、表面元素组成、力学性能、细胞毒性等级、细胞相容性、组织相容性进行了研究。结果表明:当透明质酸的含量为0.05%时,类人胶原蛋白-透明质酸支架的孔径比较均匀,孔隙率达94.38%,应力为(1000.8±7.9
3、)kPa,爆破压力为(1058.6±8.2)kPa,细胞毒性实验合格,同时具有良好的细胞相容性、组织相容性及降解性能。关键词:类人胶原蛋白,透明质酸,血管支架,生物相容性Characterizationandbiocompatibilityofhuman-likecollagen-hyaluronicacidscaffoldforbloodvessel1,31,222232XiujuanSun,DaidiFan,ChenhuiZhu,XiaoxuanMa,Yan’eLuo,LanChen,andJiaqingGuo1ShaanxiKeyLaboratoryofDe
4、gradableBiomaterials,NorthwestUniversity,Xi’an710069,China2BiomaterialsandFermentationEngineeringCenterofShaanxi,Xi’an710069,China3FacultyofLifeScience,NorthwestUniversity,Xi’an710069,ChinaAbstract:Human-likecollagen(HLC)wascross-linkedwithhyaluronicacidbygenipinindifferentratio.Theco
5、ncentrationsofhyaluronicacidinthemixturewere0,0.01%,0.05%and0.1%.Thebloodvesseltubulargraftswerethenfabricatedbyfreeze-drying.Microstructure,elementcomposite,mechanicalproperties,cytotoxicitygrade,andbiocompatibilityofdifferentvascularscaffoldgroupswerestudiedbyscanningelectronmicrosc
6、ope(SEM),X-rayphotoelectronspectroscopy(XPS),tensiletest,burstpressureexperiment,cytotoxicityexperiment,endothelialcellsplantedinbloodvesselscaffoldsandhypodermicembeddingofmice.TheresultsshowedthatHLC-HA(0.05%)tubularscaffoldexhibitedinterconnectedwell-distributedandporousstructurean
7、dporosityof94.38%;achievedthedesirablemechanicalpropertywithstressof(1000.8±7.9)kPaandburstpressureof(1058.6±8.2)kPa,hypocytotoxicity,favourablecytocompatibility,hisocompatibilityanddispositionofdegradation.Keywords:human-likecollagen,hyaluronicacid,bloodvesselscaffold,biocompatibilit
8、yRece
