天山雪莲花青素生物合成关键酶基因的克隆及功能鉴定

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1、Meanwhile,thelowconcentration(0.5mg/L-2mg/L)ofhygromycincanenhancetherateofcallusdifferentiation.CarbenidillinisanantibacterialagentofAgrobacterium,carbenidillinhadapparentlyinfluncedongrowthofcallusatdifferentconcentrations,andwhentheconcentrationofcarbenidillinwasgreaterthan400mg/

2、L,thegrowthofcalluswaseffectedobservably.TheplanttransformationvectorspMON530-DFRandpCAMBIA1305.1-3GTwereconstructedinrespective,whichcontainedcauliflowermosaicvirus(CaMV)35Spromoter,DFRgeneand3GTgene.Theresultshowedthat10minandconcentrationofAgrobacteriumwithdifferentplasmidsDNAat1

3、:1werethebestconditionfortransformation.3.GeneticTransformationofDFRand3GTinS.involucrateTheDFRand3GTofS.involucrateweretransferredrespctivelyandcollectivelyintoS.involucrateusedanAgrobacteriumrihizogenes-mediatedtransformationsystemandalargeamountofkanamycin-resistantandhygromycin-

4、resistantcalluswereobtained.ThetransformationefficienciesofDFRgenewas12.67%,3GTgenewas9.33%andcorotationofthesetwogeneswas2.63%.TheresultsonUVspectrophotometryshowedS.involucratecallusaftersuspensionculturethatanaverageoftotalflavonoidsontransgenicofDFRgenecalluswas1.86timeshigherth

5、annon-transgeniccallus,3GTgenewas2.06timeshigherthannon-transgeniccallus,corotationofthesetwogeneswas3.61timeshigherthannon-transgeniccallus.Keywords:Saussureainvolucrate,Anthocyanins,Dihydroflavonol-4-reductasegene,Flavonoid-3-O-glucosyltransferasgene,Homoosis,FlavonoidsIV英文缩写及中文对照

6、表英文缩写英文全称中文名称2,4-D2,4-DiehloroPhenoxyacetieacid2,4-苯氧乙酸2-HIS2-hydroxyisoflavanonesynthase异黄酮合酶UDP-glucose-flavonoid3GT3-O-glucosyltransferase类黄酮3-O-糖基转移酶4CL4-coumarate4-香豆酸CoA连接酶UDP-glucose-flavonoid5GT5-O-glucosyltransferase类黄酮5-O-糖基转移酶13C-NMR13C-nuclearmagneticresonance13C-核磁共振Amp

7、ampicillin氨苄青霉素ANSFlavonesynthase类黄酮合成酶ANRAnthocyanidinreductase花青素还原酶ATHydroxycinnamoylCoA肉桂酰羟基辅酶ABA6-Benzylaminopurine6-苄基腺嘌呤B5GamborgB5基本培养基bpbasepairs碱基对carCarbenidillin羧苄青霉素CHSChalconesynthase查儿酮合酶CHIChalconeisomerase查儿酮异构酶C4HCinnamate4-hydroxylase肉桂酸-4-羟化酶DFRDibydroflavonol4-r

8、eductase二氢黄酮醇还原酶DHK