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ID:32851347
大小:2.05 MB
页数:34页
时间:2019-02-16
《莪术醇对人脑胶质瘤细胞增殖与凋亡影响的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、河北医科大学硕士学位论文莪术醇对人脑胶质瘤细胞增殖与凋亡影响的实验研究姓名:秦铁城申请学位级别:硕士专业:外科学指导教师:王伟刚201203中文摘要莪术醇对人脑胶质瘤细胞增殖与凋亡影响的实验研究摘要目的:脑胶质瘤是成人原发脑肿瘤中最常见的类型,大约占到原发性恶性脑肿瘤的70%左右。目前治疗以手术辅以放化疗为主,但治疗效果有限,总体预后改善甚微。因而包括中草药在内的多种治疗手段的相关研究仍在不断深入。一般认为恶性肿瘤的根本特征在于其恶性增殖与逃避凋亡,胶质瘤自不例外。本研究观察中药活性成分莪术醇对人脑胶质瘤细胞株U251增殖与凋亡的影响,检测
2、其对胶质瘤细胞凋亡相关基因表达的影响,初步探讨其抗胶质瘤的可能机制,为脑胶质瘤的中草药治疗积累实验数据。方法:以体外培养的人脑胶质瘤细胞株U251为模型,以培养基稀释莪术醇成不同浓度,观察与检测以下内容:1MTT法检测不同时间、不同浓度莪术醇对U251细胞增殖的影响;2显微镜观察不同浓度莪术醇对U251细胞形态学的影响;3流式细胞仪检测不同浓度莪术醇对U251细胞凋亡的影响;4RT-PCR法检测不同浓度莪术醇对U251细胞中凋亡相关基因bcl.2与COX.2表达水平的影响。结果:1莪术醇对人脑胶质瘤U251细胞增殖有抑制效应,且表现出浓度依
3、赖性与时间依赖性(P4、:莪术醇;胶质瘤;凋亡;bcl一2;COX一2英文摘要Theeffectexperimentationofcurcumolonproliferationand■一-●●一一apoptoslsolhUmangliomacellABSTRACTObjective:Humangliomaisthemostcommonprimarytumorofbrain.Malignantgliomasaccountforapproximately70%ofallthenewcasesofmalignantprimarybraintumorsthataledia5、gnosedinadultseachyear.Atpresent,thestandardtreatmentconsistsofsurgicalresection,radiotherapyandchemotherapy.However,themultimodalitytherapyhasachievedonlymodestgainsformalignantgliomas.Itisgenerallybelievedthatthemalignantproliferationandapoptosisescapearethebasicfeatureo6、fmalignanttumorcell.ThisexperimentwasaimedtostudytheeffectofcurcumolontheproliferationandapoptosisofhumangliomaU251cells,toexamineitseffectonmRNAlevelsofbcl一2andCOX一2inU251cells,andtoinvestigatethepossiblemechanismofgliomagrowthinhibitionofcurcumol,andtoacumulateexperiment7、aldatafortraditionalChinesemedicineusedtotreatglioma.Methods:WithvariousconcentrationofcurcumolandhumangliomaU251cells,weobservedandexaminedsomeparameterssuchas:1T0examinetheinfluenceofdifferentconcentrationofcurcum01ontheproliferationofhumangliomaU251cellsatseriesoftimepo8、ints.2Toobservetheinfluenceofvariousconcentrationfocurcumolonthedensityandshapeofhumangli
4、:莪术醇;胶质瘤;凋亡;bcl一2;COX一2英文摘要Theeffectexperimentationofcurcumolonproliferationand■一-●●一一apoptoslsolhUmangliomacellABSTRACTObjective:Humangliomaisthemostcommonprimarytumorofbrain.Malignantgliomasaccountforapproximately70%ofallthenewcasesofmalignantprimarybraintumorsthataledia
5、gnosedinadultseachyear.Atpresent,thestandardtreatmentconsistsofsurgicalresection,radiotherapyandchemotherapy.However,themultimodalitytherapyhasachievedonlymodestgainsformalignantgliomas.Itisgenerallybelievedthatthemalignantproliferationandapoptosisescapearethebasicfeatureo
6、fmalignanttumorcell.ThisexperimentwasaimedtostudytheeffectofcurcumolontheproliferationandapoptosisofhumangliomaU251cells,toexamineitseffectonmRNAlevelsofbcl一2andCOX一2inU251cells,andtoinvestigatethepossiblemechanismofgliomagrowthinhibitionofcurcumol,andtoacumulateexperiment
7、aldatafortraditionalChinesemedicineusedtotreatglioma.Methods:WithvariousconcentrationofcurcumolandhumangliomaU251cells,weobservedandexaminedsomeparameterssuchas:1T0examinetheinfluenceofdifferentconcentrationofcurcum01ontheproliferationofhumangliomaU251cellsatseriesoftimepo
8、ints.2Toobservetheinfluenceofvariousconcentrationfocurcumolonthedensityandshapeofhumangli
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