猕猴小梁切除术后房水引流机制的分析

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1、复旦大学硕士学位论文TheStudyofTheAqueousHumorOutflowWaysPost—。TrabeculectomyinRhesusMonkey———nleroleofconjunctivallymphaticandvenoussysteminaqueousdrainageAbstractPurposeTotestifytherelationshipbetweentheconjunctivalIymphaticandvenousdrainagesystemandaqueoushumoroutf

2、lowwayspost—trabeculectomy．Methodsandmaterials1、Objectsandgroups：6adultrhesusmonkey，4．7kgto9．8kg，12eyesinal1，Randomlychooseoneeyeforsurgeryandtheotherforcontr01．2、Surgery：limbusbasedtrabeculectomywasperformed．Theconjunctivalflapwasmade8mfrom1imbusanda3X3mmh

3、alf-thicknessscleraflapdissectionwasperformedatthe1imbus，slicingabout1×2mmtrabecular，andthendoiridotomy．10／0sutureforthescleraflap，and8／0suturefortheTenon’Scapsularandconjunctira．Surgeryposition：foureyesinsuperiorandtwoininferior—temporalarea．3、Developedinj

4、ectingexperiment：inject0．1～O．3ml1％trypanblueunderflatconjunctivaby30GneedletodisplaytheIymphaticsystem．Inject0．1～0．2ml5％fluoresceinsodiumintoanteriorchambertofollowtheaqueoushumoroutflowpathways．Thesurgerygroupunderwentfluoresceinsodiumandtrypanbluedevelope

5、rexperimentasself-controlbeforesurgery．Andcontrolgroupunderwentthesametimesstainingexperimentsastheothereyesbutwithoutsurgery．Injectingexperimentdays：twicebeforesurgerywithtwoweeksinterval，and5±2、14±2daysthenevery7±2daysaftersurgery．4、Histologicalstudy：sacr

6、ificethemonkeyaccordingtothefilteringcondition．Cuttingconjunctivaintowholemountandstainingby5’-Nase．Andtheunderconjunctivasurgerypositiontissuewasmadeintoparaffinconnectiveslice．stainedbyHE、antiVEGFR-3andantiCD34inordertostudythedistributionof1ymphaticvesse

7、lSandbloodvesselsinsurgeryarea，alSOtodefinesomevesselsappearedaftersurgery．-7．复旦大学硕士学位论文Resultsl、Surgeryresults：2of6eyeshavemildTyndallphenomenaandweredisappeared7daysaftersurgery．Thefilteringblebweresustainedfor5～16daysand12．4±4．3daysinaverageundersplitlam

8、p。andlastedfor5～26．5days．16．2--+9．8dinaveragebyanteriorchamberfluoresceinsodiuminjectingexperiment．Filteringwerelast19．5～122days，49．7±43．5daysonaverageaftersurgery．Thesuturewereabsorbedorbrushedoff26．1