鞘注神经生长因子促进大鼠胫骨骨折愈合的实验分析

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1、硕士学位论文EffectsofintrathecaladministrationofnervegrowthfactoronthehealingoftibialshaftfractureintheratName：CaoLiangGuoSuperviseWangGangervlsor：anRABSTRACTBackgroundNowadays，谢tllthedevelopmentofmodernmedicine，thenutritionalinteractionbetweenneuronandnon-neurontissueshasbeenre

2、cognizedextensively．InclinicalInstitutionsapatientwhogotaTraumaticBrainInjury(TBI)accompaniedwithalong-bonefractureisverycommon．Inthesepatientstheprocessofbonehealingwerefrequentlypromotedwithalargequantityofcallus，andaheterotopicossificationsometimesasaresult．Howthecentra

3、lnervesdoescontrolthefracturehealingisuncertain．Thenervegrowthfactor(NGF)andneuropeptidedistributeextensivelyinkindsoftissues．ResultsinforeignresearchesdemonstratedthattheNGFfromendogenousorexogenouscallalsostimulatethereleaseofsensoryneuropeptidesinspinaldorsalrootganglia

4、(DRG)，changingthebiologicalactivitiesindifferentsystem．WethereforepresumedthattheNGFandneuropeptidemaybecomposedageneralregulationpathwaybetweencentralnerveandnon·neuralsystem．‘‘Dosethismechanismcontributetofracturehealing?’’SeldomreportshasbeenfoundbynOW．objective：Toexami

5、nedtheeffectofintrathecaladministrationofNGFonhealingoftibialfractureinratsandanalyzetheexpressionofcalcitoningene-relatedpeptide(CGRP)andsubstanceP(SP)．TofindtheregulationmechanismbetweencentralnerveandABSTRACTnon—neuralsystemandtodemonstratethehypothesisbasedontherelatio

6、nshipbetweenNGFandneuropeptide．Methods1．AnimalinexperimentForty-eightadultmaleSDratswererandomizedinto2equalgroups：theNGFtreatedgroupandcontrols．Ineachgroup，foursubgroupswe他establishedseparatelyaccordingtotheobservationspots(尹，14m，21髓，28mday)afteroperation．2．Experimentalmo

7、delAllofratsinthestudyhadreceivedanartificialfracture011midshaftinrighttibia．Ak-wirewasinusedtofixthefractureintramedully．Usingaspecialmicroinjector,2．5SmNGFwasperfusedthoughPEl0micro-pipeintotheintrathecalspaceattheLilevelofvertebraefor2weeks(1pg／day)．EqualvolumeofsalineW

8、asinjectedtothecontrols．3．BonecallusevaluationAt7m，14m，21n，28mdayafterNGFinjection,theani