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1、SordariaPictures,complimentsofMacieSchickandthecoolLSRIcameraClassresultsfor10/9/07-mostlylab2-lab1canusethisdata;ifyoudidnotfindanyhybridasci,thenanalyzethepicturesbelowforyourdata;addittotheclassdataLab2classdataNone112+:2g:2+:2g112+:4g:2+42g:4+:2g6Weird2Lab2872603310RightsideLeftsideLab1Instruc
2、tionsforPCRLabof10/16-10/23Herearesomeusefulwebsites:http://www.calpoly.edu/~bio/ubl/Alu.doc;http://www-hhmi.princeton.edu/Manual/cr_D1S80_pcr.docHerearetheresultsfromyourgels.Itappearsthat10of17hadDNAthatamplifiedinlab1,andlab2had7of18thatamplified.Aswediscussedinclass,theD1S80locusisoneoftheplac
3、eswithVNTR’s.Therearemanydifferentpossiblealleles.YourassignmentistoproduceareportonthisPCRexercise.Includethefollowinginyourreport:Introduction:brieflydescribethepurposeofthislab,theprincipleofPCR,andtheprincipleofidentifyingapersonbasedonPCR.Procedure:IndicatethatDNAwasobtainedfromsalivasamplesf
4、romtheclass,andamplifiedbyprimersforD1S80andseparatedbyagarosegelelectrophoresis.Summarizethesamplingprocedure,andthePCRprocedure.Includethefollowinginformationaboutthegel:Itwasa1.5%agarosegel,runforabout2hoursat75volts.TheDNAwasamplifiedwith30cycles:Denaturing:94oC,1minAnnealing:68oC,1minElongati
5、on:72oC,1min.Results:GivethetheresultsofyourPCR-ifyoudon’trememberwhichlanewasyours,Icantellyou.IndicatewhetheryouareheterozygousorhomozygousattheD1S80locus,andthesizeofthebandsthatyouproduced.Ifyoudidnotgetbands,giveresultsforLanenineoflab1,orthe“crimescene”DNAforlab2(Lane9onboththeleftandrightge
6、l).Howmanydifferentgenotypesdidyoufindforthetwolabs?DeterminingthesizeofDNA:WewillusethesizestandardsandExceltoproduceagraphofthedistancemigratedfromthewell(inmm)(Xaxis)vssizeofDNA(Yaxis).TherateatwhichDNAmigratesinagelisinverselyproportionaltothelogofitssize(usuallyinbasepairsorkilobasepairsofDNA
7、);thismeansthatsmallDNAfragmentsmigratefasterthanlargeDNAfragments,andifafragmentof100bpmigratesat40mm/hourinanelectricfield,thenafragmentof1000bpwouldmigrateat26mm/hourinthesamefield-don’tworryaboutthemath.After
