多色荧光微流控芯片电泳仪检测光路设计与实现

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2、alyticals印arationtecllIliquetllatisanaltemativetomore昀ditionalmetllodssuchaScapilla巧elec仃ophoreSis(CE)，gelelec仃ophoresis(GE)and1iquidchromato孕aphy(LC)．111meelec的phoresis，s啪plesbindillg谢tllnuorescenceares印aratedallddetected，mal(ingtlleopticalfluorescencedetectingunita访talpa

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4、onofthefourfluorescencesc戤loccllr0nlyusingf-ourdi彘reIltPMTs，increaSi芏lgitSsizeandcost．Someo协ersystemsusegmtingSasmedispersioncomponentalong谢mcustom—madeAPDs．Butt11elowe伍cientofgratingwill1eadto10wsigIlalnoisemtio(SNR)andmecustom-desigIledAPDs丽nalso1imitmeirapplicationsduet

5、o吐lehi曲costandllIlchaIlgeablestmcn鹏．NowadayS，CCDsaremore谢dely印pliediIlbiology，chemis白吼aSwellas也eelectrophoresis．F1uorescencedetectionsystemsWhichmakeuseofCCDshaveshoⅥ，11upinrecentyears．HoweVer，Ⅱle印plicationsofCCDshavebeen1imitedinMEbecauseof也e10wsensitiVi吼compared讯mPMTsorA

6、-PDs．ThuswedevelopedanuorescencespecmndetectionsystemusillgprismasmedispersioncoInponent，iIlordertoi11creaSemesensiti啊够oftheCCD．baseddetectionsyStem．h1detail，researchworkinmistllesismcludes：(1)111isthesishasdonemesimulationi11Zema)【，investigatingthe1i11eardisperSion、)l，imr

7、especttoapexan91eofmeprisma11dt11eincidentan91e．Thes妇ulationresultsi11dicatet11atusillgprismaSt11edispersioncomponentisf．easibleformenmltic010rnuorescencespec仇lmdetectionsystem．(2)Tllisthesishasdesi印ed，builtaIlddebuggedtlle1aser-inducednuorescencedetectionsyStemforME，usiIl

8、gtlleprismandCCD．Besides，t11e仃ansfonllationalgo珊吼舶mspec仇lmiInagetospec打umisi11仃oducedandS