凋亡细胞诱导免疫负相调控及其相关机制研究

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2、thapoptoticcellsw嬲d印endentontheactiVationofp38MAPKa11drec印torCD36，锄dcell．cellcontact，butnotphagocytosis．Theprimaryaimoftllisstlldyist0inVestigatewhetherapoptoticcellscouldnegatiVelyregulateimmuneresponseaIldinn锄mationbominvi仃DandinViVo，锄difso，whatismeund刚)，ingmech锄ism．Tlherefore，i

3、nthisstudy'weperf．0rmedinviVoexp甜mentsbyuSing印optoticcellsstainedby5，6一carboxylluoresceindiacetateSuccimidylestcr(CFSE)．Ourresultsshowedt11attheiIl、，iVotransfe丌ed印optoticcellswerequiCklycaptu硼(wimin4h)byhostmacrophagesinvivo．Tlle印optoticcellsd嘶Ved矗-0mtheactiVatedsplenoc”cSorbloodl

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5、sultssuggestedmattllesitewhereapoptoticcellswerephagocytosedw硒detenninedbynotonlyinmsionpamway，butalsoceUactiVationstatebefore印optosis．TheIlweinhibitedtheactiVationofKup仃ercellsin啊V0wiminViV0ad】minisn嘶onofGadolini啪Cllloride(GdCl3)orcytochalaSin，ordeletedCD11c+DCinvivowitlldiphmeri

6、atoxininvivooftheCD11c—DTRmodelmicetotestwhichkindofcellsresponsiblefortlleclear锄ceoftheinmsedapoptoticcells．(hIrresultsshowedmatmacrophag懿inspleenaIldKup伺衙cellsinliVeraremem旬orphagoc”esduringcle撕ngof印optoticcellsin、，iVo．，Wbcomparcdtllec印acityofSpl“cmacrophages，p耐tonealtbjo百ycolla

7、te—elicitedmacmphagesandKupfI’盯cellstopha90cytose印叩toticcellsinVitro，aJldfoundthatKupfrhcellsdisplayedmoreprofoundlyc印aci妙toengulfapoptoticcells．Zh柚gMGInStitIlteofImmunol99y'Zh巧i锄gUniVersi哆schoolofMedicine’H锄g办ou，310058，P．I乙Chinal2008浙江大学博上学位论文plastic．ThenwewondeI-ediftheinteracti

8、onwiⅡlapoptoticcellscouldmodi矽phe