基质细胞衍生因子-1α在创面愈合中的表达及作用研究

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1、重庆医科大学硕士研究生学位论文Methods：Fibroblastsofadultmiceskin。wereisolatedandcultured，thenweretreatedwithdifferentconcentrationsofTNF．0【andIL—l(O．1ng／mL、lOng／mL、lOOng／mL)for48h，respectively，butthecontrolgroupwastreatedwithoutthetwoagents．RT-PCRanalysiswasusedformeasuretheSDF-lamRNAexpr

2、ession．Results：SDF—lawasconstitutivelyexpressedinadultmouseskinfibroblast．ThelOWconcentrationofTNF．仅andIL．1treatmentcouldeffectivelyinhibitSDF—lamRNAexpression(P

3、tdifferenceatidemicalconcentrationbetweenTNF-0【groupsandIL-1groups(P>0．05)．Conclusions：Pro—inflammatorycytokines(n师一0【andIL-1)couldinhibittheSDF—lageneexpression-offibroblast，andtheinhibitionwasnotableinadose—dependentfashion．KeyWords：Fibroblast，TNF一0【，IL一1，Stromalcellderiv

4、edfactor—laPARTTHREEEFFECTSoFSDF．1aDURINGCUTANEoUSWOUNDHEALINGINMICEI：InductionofSDF．1aonbonemarrowderivedcellsrecruitmentduringcutaneouswoundhealing9重庆医科大学硕士研究生学位论文Objective：ToexploretheeffectofSDF-laoninducingbonemarrowderivedcells(BMDCs)recruitmenttowoundarea．Methods：BMD

5、Cswereisolatedfrombonemarrowandculturedwithroutinemethod，thenwereidentitiedbyCXCR4antibody．CellstreatmentwithCXCR4antibody(100ng／nm)for6hourswerelabeledwithChloromethyl一1，1'-dioctadecyl-3，3，3’，3'-tetramethylindocarbocyanineper—chlorate(CM-DiI)，thenwereinjectedintothetailvei

6、noffull—thicknessincisionalwoundmodelasCXCR4antibodygroup．BMDCslabeledwithCM。DilwithoutantibodytreatmentWasinjectedasBMDCsgroup，andinjectedDulbecco’SmodifiedEagle’Smediurn／F12(DMEM／F12)serum—freemediumwasascontrolgroup．ThequantityoflabeledBMDCsatthewoundsiteandthepercentage

7、ofwoundclosureweremeasured．Fouranimalsforeachexperimentaltimepointwerecollected．Results：(1)AllBMDCswereexpressedCXCR4．(2)ThepercentagesofwoundclosureofBMDCsgroupwerehigherthanthatofcontrolgroupandanti—CXCR4groupondays7and14(P<0．05)，butthepercentageofwoundclosureofanti—CXCR4

8、groupwashigherthanthatofcontrolgrouponday14(P<0．05)．(3)ThequantitiesofCM—DiIlabele