Flow-Cytometry.pdf

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1、FlowCytometryipl.qxd11/12/0611:14PageiIntroductiontoFlowCytometryPrinciplesDataanalysisProtocolsTroubleshootingByMishaRahman,Ph.D.TechnicaladvisorsAndyLane,Ph.D.AngieSwindell,M.Sc.SarahBartram,B.Sc.Yourfirstchoiceforantibodies!FlowCytometryipl.qxd11/12/0611:14PageiiFlowCytometryi

2、pl.qxd11/12/0611:14PageiiiIntroductiontoFlowCytometryPrinciplesDataanalysisProtocolsTroubleshootingByMishaRahman,Ph.D.TechnicaladvisorsAndyLane,Ph.D.AngieSwindell,M.Sc.SarahBartram,B.Sc.FlowCytometryipl.qxd11/12/0611:14Page2PrefaceHowcanIexplainwhatflowcytometryistosomeonethatkno

3、wsnothingaboutit?Well,imagineittobealotlikevisitingasupermarket.Youchoosethegoodsyouwantandtakethemtothecashier.Usuallyyouhavetopilethemontoaconveyor.Theclerkpicksuponeitematatimeandinterrogatesitwithalasertoreadthebarcode.Onceidentifiedandifsenseprevails,similargoodsarecollected

4、togethere.g.fruitandvegetablesgointooneshoppingbagandhouseholdgoodsintoanother.Nowpictureinyourmindthewholeprocessautomated;replaceshoppingwithbiologicalcells;andsubstitutethebarcodewithcellularmarkers–welcometotheworldofflowcytometryandcellsorting!Weaimtogiveyouabasicoverviewofa

5、lltheimportantfacetsofflowcytometrywithoutdelvingtoodeeplyintothecomplexmathematicsandphysicsbehinditall.Forthatthereareotherbooks(somerecommendedattheback).Instead,wepresentaguidethatwillbeinvaluabletobeginnersofflowcytometryoractasafact-packedsynopsisforthoseofyouinterestedinte

6、achingothersaboutthevirtuesofthispowerfulapplication.AtAbDSerotec,wespecializeinflowcytometryreagentsandofferthelargestrangeofantibodymarkerscommerciallyavailable,togetherwithaccessorybuffers.Ourguidingprincipleistomanufactureproductsforusewitheveryknownflowcytometryinstrument.We

7、offernobiastowardsonlyonemachinebutaremoreinterestedinmaximizingscientificperformance.Confidenceinourreagentsisshownbyourqualitypromisethatstatesthatifanyproductdoesnotperformasdescribedonitsdatasheetwewillreplacethereagentwithafreshvialorofferyoucredit.Pleasecontactusorviewourwe

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