transgenic animal.pdf

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1、LectureOutlineßIntroduction(briefhistoryoftransgenicanimals(timelineofanimalcloningandgenetransfer)ßGenetransfertechniquesßScreening&characterizationoftransgenicsTransgenicAnimalsßApplicationsoftrasngenicanimals-Custom-madeanimals-Animalbioreactors-Buildi

2、ngabetteranimal-EnvironmentalreplacementsDr.J.K.Lu陸振岡•Trasngenicaquacticorganisms•BiosafetyofGManimals•Futureaspects1-11-2006BriefhistoryofgenetransferBriefhistoryoftransgenicanimals1891firstsuccessfulembryotransferearly1900’sinvitroembryo(continue)cultur

3、edevelop1961Mouseembryoaggregationtoproducechimeras1966firstreportofmicrioinjectionofmouseembryos1987chimeric“knockout”micedescribed1973foreigngenefunctionaftercelltransfection1987retrovirusmediatedtransgenicchicken1974developmenttoteratocarcinomacelltran

4、sfer1989targetedDNAintegration&germlinechimericmiceR.JansishmicroinjectedMMLVgeneintomouseembryos1989firstsperm-mediatedreportsfarmanimals1977mRNA&DNAtransferredtoXenopouseggs1980mRNAtrasnferredintomammalianova1991microinjectionfortransgenicgoatsfirstrepo

5、rted1980-1981transgenicmicefirstdocumented1993germlinechimericmiceproducedusingco-culture1981transferEScellsderivedfrommouseembryos1996EScellsusedfornucleartransfersheep1982transgenicmiceandagrowthhormonephenotype1983tissuespecificgeneexpressionintransgen

6、icmice1997somaticcellfromadultsheepusedforclonningby1984transgenicdrawfmicecarryinghGHgrowingsizenormallynucleartransfer(Dolly)1985transgenicdomesticanimalsproduced1987microinjectionfortransgeni1987microinjectionfortransgeniccpigs,sheep,rabbits,fishpigs,s

7、heep,rabbits,fishTransgenicMiceMilestoneofsometransgenicMicroinjectionresearches1980-1981transgenicmicefirstdocumented:GiganticMice¾J.Gordon&F.RuddlemicroinjectDNAintomalepronucleus(1980;1981)¾R.Brinsterinjectviralgene(TK)intomousepronucleus&detectedforei

8、gnDNAexpression(1981)Applications:¾F.Contantini&E.Lacyinjectrabbitβ–globin•Tissue-specificgeneexpressiongeneintomouseembryoandintegratedinto•Physiologicalconsequenceimpactsgermline(1981)•Oncogenestudy*Thesestudiessu