儿童过敏性紫癜外周血单个核细胞tlr2和myd88 mrna表达初步观察

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1、儿童过敏性紫癜外周血单个核细胞TLR2和MyD88mRNA表达初步观察摘要目的探讨Toll样受体2(Toll—likereceptor2，TLR2)、髓样细胞分化蛋白88(Myeloiddifferentiationprimaryresponsegene88，MyD88)、肿瘤坏死因子a(TumorNecrosisFactoralpha，TNF—a)mRNA在过敏性紫癜(Henoch—SchSnleinpurpura，HSP)患儿外周血单个核细胞的表达，从基因水平揭示TLR2、MyD88、TNF-Q在HSP患儿的免疫发病机制中的可能作用。方法选取2011年11月至201

2、2年11月就诊于青岛大学医学院附属医院急性期HSP患儿64例为病例组，其中合并肾损害HSP组28例，无肾损害HSP组36例，健康对照组为我院健康体检儿童30例。无菌条件下采用密度梯度离心法获取外周血单个核细胞(Peripheralbloodmononuciearcells，PBMC)，采用荧光定量PCR检测外周血单个核细胞TLR2、MyD88、TNF-ⅡmRNA的表达。两组间比较采用t检验，多组间比较采用单因素方差分析和LSD～t检验，TLR2、MyD88、TNF—QmRNA表达变化相关性采用spearman秩相关分析。结果(1)HSP患儿PBMCTLR2、MyD88和

3、TNF-QmRNA表达量与正常对照组比较均显著增高(pO．05)。结论HSP患儿PBMcTLR2、M

4、yD88、TNF-dmRNA表达明显升高，有肾损害组也明显高于无肾损害组，提示PBMCTLR2和MyDSSmRNA的过表达可能参与HSP和紫癜性肾炎的免疫发病机制。硕士研究生指导教师孙燕(儿科学)张秋业教授关键词：过敏性紫癜；Toll样受体2；髓样细胞分化蛋白88；肿瘤坏死因子一aTheexpressionpreliminaryobservationofTLR2，MyD88andTNF—amRNAfromPeripheralbloodmononuclearcellsinchildrenwithHenoch—SchSnleinpurpuraAbstractObjectiv

5、eTorevealthepossibleroleofToll—likereceptor2,Myeloiddifferentiationprimaryresponsegene88andTumorNecrosisFactoralphainimmunologicalpathogenesisofHenoch—SchOnleinpurpurafromgenelevelbystudyingtheexpressionlevelsofTLR2．MyD88andn、IF吨mRNAfromperipheralbloodmononuclearcellsinchildrenwithHenoch

6、-·SchOnleinpurpura．Methodssixty-fourHSPpatientsinacutephaseweresubsequentlyenteredintostudygroupwhowerehospitalizedinDepartmentofPediatrics，TheAffiliatedHospitalofQingdaoUniversityMedicalCollage，fromNovember201toNovember2012．TheHSPgroupWasdividedintotwogroups，includingpatients谢threnaldam

7、age(n236)andthosewithoutrenaldamage(n-28)．№heahbychildrenconstitutedthecontrolgroupwhowerecarriedonthehealthycheck-upinthehospital．Peripheralbloodmononuclearcells(PBMC)wereisolatedbydensitygradientcentrifugationmethodinasepticconditions．Real-timefluorescentquantitativepol