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ID:34351848
大小:2.95 MB
页数:53页
时间:2019-03-05
《抗苗勒管激素及其ⅱ型受体与多囊卵巢综合征卵母细胞成熟的关系》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、抗苗勒管激素及其Ⅱ型受体与多囊卵巢综合征卵母细胞成熟的关系中文摘要目的通过研究卵胞浆内单精子显微注射(intracytoplasmicsperminjection,ICSI)周期取卵日多囊卵巢综合征患者(polycysticovariansyndrome,PCOS)与对照组血清抗苗勒管激素(anti.mullerianhormone,AMH)水平、卵泡液AMH水平、卵丘颗粒细胞抗苗勒管激素II型受体(anti.mullerianhormonetyrpeIIreceptor,AMHRII)的表达水平,探讨抗
2、苗勒管激素及其II型受体与卵母细胞成熟的关系,分析抗苗勒管激素及其II型受体对卵母细胞成熟的影响;探讨抗苗勒管激素及其II型受体与PCOS卵泡发育障碍的关系。方法选择2011年6月.2012年8月因PCOS接受ICSI治疗的20例患者为研究对象,同时选择因男方因素就诊于青岛市妇女儿童医院生殖中心需行ICSI治疗的32例不孕患者为对照组,取卵日采集3ml静脉血,一对一收集卵泡液及卵丘颗粒细胞,记录获卵数、卵子成熟度、受精情况及其胚胎质量,用酶联免疫吸附法(enzyme.1inkedimmunosorbent
3、assay,ELISA)测定血清及卵泡液内AMH水平,用TRIzol试剂盒提取卵丘颗粒细胞中总RNA,实时荧光定量聚合酶链式反应(Real.timePCR)测定卵丘颗粒细胞中AMHII型受体mRNA的表达量。结果1.PCOS组及对照组卵泡液AMH水平均高于血清AMH水平(尸4、液AMH水平(尸<0.001);PCOS组MII卵母细胞卵泡液AMH水平高于对照组MII卵母细胞,PCOS组MI卵母细胞卵泡液AMH水平高于对照组MI卵母细胞。4.PCOS组及对照组MI期卵丘颗粒细胞AMHRIImRNA表达均显著高于MII期(闷.001);PCOS组MI期卵丘颗粒细胞AMHRIImRNA表达显著高于对照组MI期;PCOS组MII期卵丘颗粒细胞AMHRIImRNA表达显著高于对照组MII期。5.对照组卵泡液AMH水平与受精率负相关(卢0.007),卵泡液内AMH水平与优胚率无相关性(尸=05、.202);PCOS组卵泡液AMH水平与受精率及优胚率无相关性结论—蝴H可能通过II型受体抑制卵母细胞成熟,与PCOS卵泡发育障碍有关;血清AMH/获卵数可以作为预测卵母细胞成熟度的参考因子。硕士研究生姜宙(妇产科学)指导教师邹淑花教授关键词:多囊卵巢综合征;抗苗勒管激素;抗苗勒管激素Ⅱ型受体;卵母细胞成熟度;卵丘颗粒细胞Relationshipbetweenanti.mullerianhormone,anti.mullerianhormonereceptortypeⅡandoocytematuratio6、nofpolycystieovariansyndromeObjectiveThisprospectivestudywastoobservethedifferenceoffollicularfluidanti—mullerianhormone(AMH),serumAMH,anti.mullerianhormonereceptortypeIIexpression(AMHRII)betweenPCOSgroupandcontrolgroup.Toinvestigatetherelationshipamongan7、ti—mullerianhormone,anti.mullerianhormonereceptortypeIIexpressionandoocytematurationwithpolycysticovariansyndrome(PCOS)ofoocytesduringintracytoplasmicsperminjection(ICSI)cycle.MethodsCumulusgranularcellsandfollicularfluidsandbloodsampleswereobtainedfrom208、womenwithPCOSand32ovulatorywomenofcontrolgroupundergoingintracytoplasmicsperminjectiontreatmentonovumpick.uDday.A1lthesampleswererecordedoocytematurationandthenumberofretrievedoocytes.Theinfluenceofserumandfollicula
4、液AMH水平(尸<0.001);PCOS组MII卵母细胞卵泡液AMH水平高于对照组MII卵母细胞,PCOS组MI卵母细胞卵泡液AMH水平高于对照组MI卵母细胞。4.PCOS组及对照组MI期卵丘颗粒细胞AMHRIImRNA表达均显著高于MII期(闷.001);PCOS组MI期卵丘颗粒细胞AMHRIImRNA表达显著高于对照组MI期;PCOS组MII期卵丘颗粒细胞AMHRIImRNA表达显著高于对照组MII期。5.对照组卵泡液AMH水平与受精率负相关(卢0.007),卵泡液内AMH水平与优胚率无相关性(尸=0
5、.202);PCOS组卵泡液AMH水平与受精率及优胚率无相关性结论—蝴H可能通过II型受体抑制卵母细胞成熟,与PCOS卵泡发育障碍有关;血清AMH/获卵数可以作为预测卵母细胞成熟度的参考因子。硕士研究生姜宙(妇产科学)指导教师邹淑花教授关键词:多囊卵巢综合征;抗苗勒管激素;抗苗勒管激素Ⅱ型受体;卵母细胞成熟度;卵丘颗粒细胞Relationshipbetweenanti.mullerianhormone,anti.mullerianhormonereceptortypeⅡandoocytematuratio
6、nofpolycystieovariansyndromeObjectiveThisprospectivestudywastoobservethedifferenceoffollicularfluidanti—mullerianhormone(AMH),serumAMH,anti.mullerianhormonereceptortypeIIexpression(AMHRII)betweenPCOSgroupandcontrolgroup.Toinvestigatetherelationshipamongan
7、ti—mullerianhormone,anti.mullerianhormonereceptortypeIIexpressionandoocytematurationwithpolycysticovariansyndrome(PCOS)ofoocytesduringintracytoplasmicsperminjection(ICSI)cycle.MethodsCumulusgranularcellsandfollicularfluidsandbloodsampleswereobtainedfrom20
8、womenwithPCOSand32ovulatorywomenofcontrolgroupundergoingintracytoplasmicsperminjectiontreatmentonovumpick.uDday.A1lthesampleswererecordedoocytematurationandthenumberofretrievedoocytes.Theinfluenceofserumandfollicula
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