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1、第26卷第2期西安体育学院学报Vol126No122009年3月JOURNALOFXI′ANPHYSICALEDUCATIONUNIVERSITYMar12009运动人体科学文章编号:10012747Ⅹ(2009)0220206204文献标识码:A中图分类号:G80417X运动对肥胖大鼠脂肪细胞脂联素mRNA表达的影响1223李世成,邵雄杰,吴苹,刘军(1.湛江师范学院体育科学学院,广东湛江524048;2.中山大学第三医院,广州510630;3.解放军422医院,广东湛江524000)摘要:目的探讨运动对脂肪细胞脂联素mRNA表达的影响,从脂肪细胞内分泌的角度研究肥胖的机制。方法断
2、乳5天SD健康雄性幼鼠40只,体重75-85g随机分组为普通饲料喂养组和高脂饲料喂养组,高脂饲料喂养组在3周后筛选出肥胖鼠,并随机分组为不运动对照组和运动干预组。运动大鼠进行游泳运动,运动时间第1周为30min/d、第2周为60min/d(运动30min休息5min)、第3周为90min/d(运动30min休息5min)、第4周为120min/d(运动30min休息5min),每周运动6d,周日休息,共4w。实验后处死所有大鼠,取睾丸脂肪垫和腹膜后脂肪组织测试脂联素mRNA表达水平。结果肥胖大鼠运动4周后血清脂联素浓度增加了51.81%(P<0.05),睾丸脂肪垫和腹膜后脂肪组织脂联
3、素mRNA表达水平分别增加86.36%(P<0.05)和94.74%(P<0.05)。结论运动明显增高血清脂联素水平和内脏脂肪组织脂联素mRNA的表达。关键词:脂联素mRNA;运动;脂肪细胞;肥胖大鼠EffectsofAerobicExerciseonAdiponectinmRNAExpressioninAdipocyteofObeseRats1223LIShi2cheng,SHAOXiong2jie,WUPing,LIUjun(1.SchoolofPhysicalEducation,ZhanjiangNormalCollege,Zhanjiang524048,China;2.The
4、thirdAffiliatedHospitalofSunYat-senUniversity,Guangzhou510630,China;3.ThePLA422Hospital,Zhanjiang524005,China)Abstract:ObjectiveTostudytheeffectsofexerciseonexpressionofadiponectinmRNAinadipocyte,andthemechanismthatexer2ciseintervenedinobesitytobeelucidated.Methods40maleratswhoseweightwasbetwee
5、n75gand85gwereweanedforfivedaysanddividedintonormaldietandhighfatdietgroupsrandomly.After3weeks,Obeseratswerechoosedanddividedintocontrolgroupandexperimentgrouprandomly.Theratsinexperimentgroupweredealedwithswimimgtrainingfor4weeks,exercisedsixdaysperweekandrestedoneday.Exercisetimewas30minperd
6、ayinthefistweek,60minperweekinthesecondweek,90minperweekinthethirdweekand120minperweekinthelastweek.Fromsecondweek,Ratsswamfor30minandbreaked30min.After4weeks,allratswerekilledandthelevelsofadiponectinmRNAexpressionweremeasuredintestisfadpadandretroperitonealfatpadofrat.ResultsAsObeseratsexerci
7、sedin4weeks,thelevelofserumadiponectinwasincreased51.81%(P<0.05).andtheexpressionofadiponectinmRNAincreased86.36%(P<0.05)intestisfadpadandincreased94.74%(P<0.05)inretroperitonealfatpad.ConclusionsThelevelofserumadiponectinincrease
