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1、TMMETHODSINMOLECULARBIOLOGYSeriesEditorJohnM.WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForothertitlespublishedinthisseries,gotowww.springer.com/series/7651TMMETHODSINMOLECULARBIOLOGYTherapeuticApplicationsofRNAiMethodsan
2、dProtocolsEditedbyJohnF.Reidhaar-OlsonCristinaM.RondinoneHoffmann-LaRocheInc.,Nutley,NJ,USAEditorsJohnF.Reidhaar-OlsonCristinaM.RondinoneHoffmann-LaRocheInc.Hoffmann-LaRocheInc.340KingslandStreet340KingslandStreetNutleyNJ07110NutleyNJ07110USAUSAjohn.reidhaar-ols
3、on@roche.comcristina.rondinone@roche.comISSN1064-3745e-ISSN1940-6029ISBN978-1-60327-294-0e-ISBN978-1-60327-295-7DOI10.1007/978-1-60327-295-7SpringerDordrechtHeidelbergLondonNewYorkLibraryofCongressControlNumber:2009926661cHumanaPress,apartofSpringerScience+Bus
4、inessMedia,LLC2009Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsors
5、cholarlyanalysis.Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarter
6、ms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.Whiletheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateofgoingtopress,neithertheauthorsnortheeditorsnorthepublisherca
7、nacceptanylegalresponsibilityforanyerrorsoromissionsthatmaybemade.Thepublishermakesnowarranty,expressorimplied,withrespecttothematerialcontainedherein.Printedonacid-freepaperSpringerispartofSpringerScience+BusinessMedia(www.springer.com)PrefaceIntheshorttimesin
8、ceitsdiscovery,RNAinterference(RNAi)hasbecomeawell-establishedtoolinthedrugdiscoveryprocess.Theabilitytoknockdownexpressionofanygeneinmammaliancellsusingastraightforwardmeth
