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时间:2019-03-08
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1、TheEnglish-ChineseBilingualTeachingandLearningOutlineofBiochemistryⅡ(EnglishVersion)ChapteroneNucleotideandNucleicacidsPropose:1.Graspofthenucleotidestructure,includingthecomponentofthenucleotideandthechemicalbondslinkingthecomponents,andthenamesandabbreviationsofbases,nucleosidesandnu
2、cleotides.矚慫润厲钐瘗睞枥庑赖。2.ProfoundgraspofthekeyknowledgeofthestructureandfunctionofDNAandRNA.聞創沟燴鐺險爱氇谴净。3.Familiaritywiththephysio-chemicalpropertiesofDNAandtherelationbetweenthesepropertiesandtheDNAstructure.残骛楼諍锩瀨濟溆塹籟。4.ToknowthetechniqueofisolationandpurificationofnucleicacidsandDNAseq
3、uencing.酽锕极額閉镇桧猪訣锥。Contents:1.Generalconceptsofnucleicacids.(1)Nucleotidesarethebuildingblocksofnucleicacids.彈贸摄尔霁毙攬砖卤庑。(2)Nucleotideiscomposedofbase,pentose,andphosphate.謀荞抟箧飆鐸怼类蒋薔。(3)The3’,5’phosphodiesterbondlinksnucleotides,manynucleotidesarelinkedbythe3’,5’phosphodiesterbondtoform
4、nucleicacids.厦礴恳蹒骈時盡继價骚。(4)NucleicacidsaredividedintoDNAandRNA.(5)Otherfunctionsofthenucleotides.2.thethreedimensionedstructureofDNA(1)DoublehelixstructureisthesecondarystructureofDNA.茕桢广鳓鯡选块网羈泪。①ThedoublehelixDNAmodelputforthbyWastonandCrick.鹅娅尽損鹌惨歷茏鴛賴。②ThefeaturesofdoublehelixDNA.③A-
5、DNAandZ-DNA.④TriplestrandDNA.(2)SupercoilsofDNA①ProkaryoticcircularsupercoiledDNA.②EukaryoticmtDNAandctDNA.③Topoisomerases.(3)Eukaryoticchromosome①Nucleosome.②Fromchromosometochromatinandchromosome.(4)DNAisthegeneticmaterial①Avery’sexperiment.②Thegenome.③Euchromatinandheterochromatin.3
6、.StructureandfunctionsofRNA(1)FourmainkindsofRNA(2)mRNA:structureandfunction.7/7(1)tRNA:structureandfunction.(2)rRNA:structureandfunction.(3)OthersmallRNAs.2.Physico-chemicalpropertiesofnucleicacids(1)Hydrolysis.(2)UVabsorbance.(3)Denaturation,renaturation,andhybridization.5.Nuclease(1
7、)Ribonuclease.(2)Dexoyribonuclease.6.TechniquesofisolationandpurificationofnucleicacidsandDNAsequencing.籟丛妈羥为贍偾蛏练淨。(1)Methodsofisolationandpurificationofnuceicacids.預頌圣鉉儐歲龈讶骅籴。(2)DNAsequencing.ChaptertwoNucleotidemetabolismPurpose1.Toknowdigestionandabsorptionofnucleicacidsandanaboli
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