microrna-7调控胃癌恶性生物学行为的功能与分子机制研究

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1、分类号R735.2UDC616-006密级公开microRNA-7调控胃癌恶性生物学行为的功能与分子机制研究赵晓迪培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)内科学（消化系病）研究方向胃癌相关非编码RNA指导教师樊代明教授（主任医师）培养单位西京消化病医院二O一五年五月第四军医大学博士学位论文目录缩略语表·······························································································1中文

2、摘要·······························································································3Abstract·································································································7前言···························································

3、···································12文献回顾······························································································13正文······························································································25第一部分miR-7在胃癌细胞和胃癌组织中的表达模式及其临床意

4、义················251材料····························································································251.1细胞系····················································································251.2组织标本··················································

5、·······························251.3主要试剂·················································································261.4主要仪器·················································································262方法·················································

6、···········································272.1细胞培养·················································································272.2细胞和组织总RNA的提取··························································272.3实时定量PCR检测细胞和组织中miR-7的表达·····························

7、··272.4组织原位杂交检测组织芯片中miR-7的表达····································283结果····························································································303.1miR-7在胃癌细胞和胃癌组织中的表达模式····································303.2miR-7表达与胃癌分级、分期的相关性··················

8、························314讨论····························································································33第二部分miR-7在胃癌细胞恶性生物学行为中