crisprcas9定点编辑osga20ox2基因降低水稻株高研究

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1、分类号：Q78密级：公开论文编号：2013021141贵州大学2016届硕士研究生学位论文CRISPR/Cas9定点编辑OsGA20ox2基因降低水稻株高研究学科专业：生物化学与分子生物学研究方向：植物分子生物学与基因工程导师：赵德刚教授研究生：张笑寒中国﹒贵州﹒贵阳2016年6月2016MasterThesisofGuizhouUniversityMutationofOsGA20ox2genebasedonCRISPR/Cas9editingsysteminrice(OryzasativaL.)

2、Speciality:BiochemistryandMolecularBiologyResearchfield:PlantMolecularBiologyandGeneEngineeringAdvisor:Prof.De-GangZHAOCandidate:Xiao-HanZHANGGuiyangChinaJune,2016目录摘要....................................................................................

3、...................................................1Abstract.......................................................................................................................................3第一章文献综述................................................

4、.....................................................................51水稻株高调控基因研究进展.................................................................................................51.1赤霉素生物合成及信号转导途径相关基因.................................................

5、...........51.2油菜素内酯生物合成及信号转导途径相关基因....................................................71.3其他与水稻株高相关的基因....................................................................................92水稻转基因技术研究进展...................................................

6、..................................................92.1水稻遗传转化方法研究进展....................................................................................92.1.1PEG介导法.........................................................................................

7、..........102.1.2电击法..........................................................................................................102.1.3基因枪法......................................................................................................102.1.4花粉管通道

8、法..............................................................................................102.1.5农杆菌介导法..............................................................................................112.2水稻遗传转化受体............................