低剂量节拍化疗对卵巢癌细胞skov3血管生成拟态的影响及其机制的探讨

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1、授予单位代码10089学号或申请号2(H22432HebeiMedicalUniversity硕士学位论文科学学位低剂量节拍化疗对卵巢癌细胞SKOV3血管生成拟态的影响及其机制的探讨研究生：陈琳导师：吴小华教授专业：妇产科学二级学院：白求恩国际和平医院2015年3月目录中文摘要····································································································1英文摘要···············

2、·····················································································4英文缩写····································································································8研究论文低剂量节拍化疗对卵巢癌细胞SKOV3血管生成拟态的影响及其机制的探讨前言····························

3、········································································9材料与方法························································································9结果··························································································

4、········23附图··································································································25附表··································································································32讨论···········································

5、·······················································34结论··································································································36参考文献··························································································36综述肿瘤

6、血管生成及抗肿瘤血管生成治疗现状································39致谢··········································································································48个人简历·······························································································

7、···49中文摘要低剂量节拍化疗对卵巢癌细胞SKOV3血管生成拟态的影响及其机制的探讨摘要目的：血管生成拟态（vasculogenicmimicry,VM）是高侵袭性恶性肿瘤细胞所具有的自我血供的微循环结构。本实验通过对荷人卵巢上皮性癌SKOV3细胞裸鼠异种移植瘤模型行不同模式的顺铂（DDP）化疗获取移植瘤原代细胞，比较接受低剂量节拍化疗（low-dosemetronomicchemotherapy，LDM）和最大可耐受剂量化疗（maximumtolerateddosechemotherapy，MTD）后SKOV

8、3移植瘤原代细胞形成VM的能力、增殖力及侵袭力，同时分析不同化疗模式对干细胞标记物CD133、ALDH1蛋++--白表达的影响，并比较CD133ALDH1和CD133ALDH1细胞形成血管生成拟态的差异，以探讨不同模式化疗对卵巢癌SKOV3细胞形成VM的影响及其可能机制。方法：71建立裸鼠移植瘤模型并分组化疗：体外培养SKOV3细胞，将1×10个细胞接种于雌性裸鼠右侧的大